Muangchan Nipaporn, Kooptiwut Suwattanee, Tapechum Sompol, Akarasereenont Pravit, Vongsopanagul Nuanchan, Pongwattanapakin Kitchaya, Chaikomin Reawika
Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University.
Department of Pharmacology and Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University.
Biol Pharm Bull. 2017;40(9):1506-1514. doi: 10.1248/bpb.b17-00320.
Gastric motility disturbance is commonly found in long-standing hyperglycemia. Both delayed and rapid gastric emptying has been reported in diabetes. However, very few studies have followed the changes in gastric emptying during disease progression in diabetes because of technical limitations. C-Acetic acid breath test is a validated method which is non-invasive and can be used repeatedly or serially to evaluate gastric emptying changes in animal. We investigated the gastric emptying changes in different stages of diabetes using C-acetic acid breath test, as well as its related mechanisms involving interstitial cells of Cajal (ICCs), and stem cell factor (SCF) in streptozotocin-induced diabetic rats. The results showed that gastric emptying was accelerated at the early stage (12 weeks of diabetes) whereas intramuscular ICCs (ICC-IM) networks were not different from normal group. At long-term stage (28 weeks of diabetes), gastric emptying had returned to normal pattern with no delayed. ICC-IM networks were decreased in the diabetic group compared to 12th weeks, and were lower than in the normal group at the same time point. SCF levels were constantly high in the diabetic group than in the normal group. This result indicated that C-acetic acid breath test is useful to track the alteration in gastric emptying during disease progression. The change of gastric emptying was not found to be significantly associated with ICC-IM. Elevated SCF may help to preserve ICC-IM, especially in the early phase of diabetes.
胃动力障碍在长期高血糖中很常见。糖尿病患者中已报道有胃排空延迟和排空过快的情况。然而，由于技术限制，很少有研究跟踪糖尿病病情进展过程中胃排空的变化。C-乙酸呼气试验是一种经过验证的方法，它是非侵入性的，可重复或连续用于评估动物的胃排空变化。我们使用C-乙酸呼气试验研究了链脲佐菌素诱导的糖尿病大鼠在糖尿病不同阶段的胃排空变化，以及其涉及Cajal间质细胞（ICC）和干细胞因子（SCF）的相关机制。结果显示，在糖尿病早期（糖尿病12周）胃排空加速，而肌内ICC网络与正常组无差异。在长期阶段（糖尿病28周），胃排空恢复正常模式，无延迟。与第12周相比，糖尿病组的肌内ICC网络减少，且在同一时间点低于正常组。糖尿病组的SCF水平始终高于正常组。这一结果表明，C-乙酸呼气试验有助于跟踪疾病进展过程中胃排空的变化。未发现胃排空的变化与肌内ICC有显著相关性。SCF升高可能有助于维持肌内ICC，尤其是在糖尿病早期。
