Causon R, Davies D, Brown M
Life Sci. 1987 Aug 17;41(7):909-12. doi: 10.1016/0024-3205(87)90193-7.
Electrochemical detection of eluted solutes in HPLC is now well established as a selective and highly sensitive technique. Measurement of catecholamines and metabolites has been the most popular application with an exponential increase in literature. Recent improvements include the use of diphenylborate for extractions, the introduction of smaller diameter HPLC materials which improve resolution and sensitivity, and the replacement of carbon paste with glassy carbon which now gives a more stable electrode with similar sensitivity. Applications of HPLC with amperometric detection to the determination of drugs, amino acids and peptides are discussed.
高效液相色谱（HPLC）中洗脱溶质的电化学检测现已成为一种成熟的选择性和高灵敏度技术。儿茶酚胺及其代谢物的测定一直是最受欢迎的应用，相关文献呈指数级增长。最近的改进包括使用二苯硼酸盐进行萃取、引入更小直径的HPLC材料以提高分离度和灵敏度，以及用玻碳替代碳糊，如今玻碳能提供灵敏度相似但更稳定的电极。本文讨论了高效液相色谱-安培检测在药物、氨基酸和肽类测定中的应用。
