Simmons P J, Przepiorka D, Thomas E D, Torok-Storb B
Nature. 1987;328(6129):429-32. doi: 10.1038/328429a0.
Although it is generally agreed that stromal cells are important in the regulation of haematopoietic cell development, the origin of these phenotypically diverse cells has been a subject for debate for more than 50 years. Data which support the concept of a separate origin for the haematopoietic stem cell and the marrow stroma are derived from cytogenetic or enzyme marker studies of explanted and expanded stromal cells grown under conditions that do not allow haematopoiesis in vitro. Recent evidence in man and in mouse suggesting that the stromal cells capable of transferring the haematopoietic microenvironment in vitro are transplantable seemingly questions this dichotomy, one interpretation being the existence of a common haematopoietic/stromal 'stem cell'. We used in situ hybridization to discriminate donor cells from host in blood and bone marrow samples obtained from patients with functioning sex-mismatched but HLA-identical allografts. Without exception, marrow-derived stromal cells that proliferate in long-term cultures were found to be of host genotype, whereas the macrophage component of the adherent layer in these cultures originated from the donor.
尽管人们普遍认为基质细胞在造血细胞发育的调节中很重要，但这些表型多样的细胞的起源在50多年来一直是一个争论的话题。支持造血干细胞和骨髓基质细胞有独立起源这一概念的数据，来自于对在体外不允许造血的条件下培养和扩增的基质细胞进行的细胞遗传学或酶标记研究。最近在人和小鼠中的证据表明，能够在体外传递造血微环境的基质细胞是可移植的，这似乎对这种二分法提出了质疑，一种解释是存在一种共同的造血/基质“干细胞”。我们使用原位杂交技术，在从具有功能的性别不匹配但HLA相同的同种异体移植患者获得的血液和骨髓样本中区分供体细胞和宿主细胞。无一例外，在长期培养中增殖的骨髓来源的基质细胞被发现是宿主基因型，而这些培养物中贴壁层的巨噬细胞成分则来自供体。
