Yeh Hsien-Wei, Karmach Omran, Ji Ao, Carter David, Martins-Green Manuela M, Ai Hui-Wang
Department of Chemistry, University of California Riverside, Riverside, California, USA.
Department of Molecular Physiology and Biological Physics and Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400. Epub 2017 Sep 4.
Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.
红移生物发光报告基因对于生物成像很有必要。我们描述了基于合成腔肠素类似物和相应的纳米荧光素酶突变体开发的红移荧光素，其可实现明亮的生物发光。特别是一对荧光素在体外和体内表现出优于常用生物发光报告基因的灵敏度。我们采用这一对荧光素开发了一种基于生物发光共振能量的Antares报告基因，称为Antares2，它能增强来自深部组织的信号。
