Vora Moiz, Alattia Lubna A, Ansari Junaid, Ong Menchu, Cotelingam James, Coppola Domenico, Shackelford Rodney
Department of Pathology and Translational Pathobiology, LSU Health, Shreveport, LA, U.S.A.
Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, LA, U.S.A.
Anticancer Res. 2017 Sep;37(9):4821-4825. doi: 10.21873/anticanres.11889.
BACKGROUND/AIM: Nicotinamide phosphoribosyl transferase (Nampt) catalyses the rate-limiting step of the mammalian nicotinamide adenine dinucleotide (NAD) salvage pathway. Nampt is highly expressed in several epithelial and mesenchymal neoplasms, where is promotes cell-cycle progression ans chemotherapy resistance. To our knowledge, alterations in Nampt expression have not been examined in cervical intraepithelial neoplasia (CIN) or squamous cell carcinoma (SCC).
We performed immunohistochemical analysis for Nampt using tissue microarrays on 14 samples of benign cervical squamous epithelium and 15 CIN I, 15 CIN II, and 13 samples of CIN III. The SCCs included 5 low-grade, 67 intermediate-grade, and 81 high-grade tumors.
Nampt levels increased with increased CIN grades were compared to benign cervical squamous epithelium. Similarly, Nampt levels increased with increasing SCC grade.
Nampt expression is a reliable marker of progression in cervical dysplasia and SCC.
背景/目的：烟酰胺磷酸核糖转移酶（Nampt）催化哺乳动物烟酰胺腺嘌呤二核苷酸（NAD）补救途径的限速步骤。Nampt在几种上皮和间充质肿瘤中高度表达，在这些肿瘤中它促进细胞周期进程和化疗耐药性。据我们所知，尚未在宫颈上皮内瘤变（CIN）或鳞状细胞癌（SCC）中检测Nampt表达的改变。
我们使用组织微阵列对14例良性宫颈鳞状上皮样本、15例CIN I、15例CIN II和13例CIN III样本进行了Nampt的免疫组织化学分析。SCC包括5例低级别、67例中级别和81例高级别肿瘤。
与良性宫颈鳞状上皮相比，Nampt水平随CIN级别增加而升高。同样，Nampt水平随SCC级别增加而升高。
Nampt表达是宫颈发育异常和SCC进展的可靠标志物。
