Hefter Maike, Lother Jasmin, Weiß Esther, Schmitt Anna Lena, Fliesser Mirjam, Einsele Hermann, Loeffler Juergen
Medizinische Klinik und Poliklinik II, University Hospital Wuerzburg, Wuerzburg, Germany.
Med Mycol. 2017 Jul 1;55(5):573-578. doi: 10.1093/mmy/myw105.
Aspergillus fumigatus is an opportunistic fungal pathogen causing detrimental infections in immunocompromised individuals. Dendritic cells (DCs) are potent antigen-presenting cells and recognize the A. fumigatus cell wall component β-1,3 glucan via Dectin-1, followed by DC maturation and cytokine release. Here, we demonstrate that human primary myeloid DCs (mDCs) interact with different morphotypes of A. fumigatus. Dectin-1 is expressed on mDCs and is down-regulated after contact with A. fumigatus, indicating that mDCs recognize A. fumigatus via this receptor. Blocking of Dectin-1, followed by stimulation with depleted zymosan diminished the up-regulation of the T-cell co-stimulatory molecules CD40, CD80, HLA-DR and CCR7 on mDCs and led to decreased release of the cytokines TNF-α, IL-8, IL-1β and IL-10.
烟曲霉是一种机会性真菌病原体，可在免疫功能低下的个体中引起有害感染。树突状细胞（DCs）是强大的抗原呈递细胞，可通过Dectin-1识别烟曲霉细胞壁成分β-1,3-葡聚糖，随后DC成熟并释放细胞因子。在此，我们证明人类原代髓样DCs（mDCs）与烟曲霉的不同形态型相互作用。Dectin-1在mDCs上表达，与烟曲霉接触后下调，表明mDCs通过该受体识别烟曲霉。阻断Dectin-1，然后用脱毒酵母聚糖刺激，可减少mDCs上T细胞共刺激分子CD40、CD80、HLA-DR和CCR7的上调，并导致细胞因子TNF-α、IL-8、IL-1β和IL-10的释放减少。
