College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.
Department of Entomology, China Agricultural University, Beijing, 100094, China.
Sci Rep. 2017 Sep 5;7(1):10429. doi: 10.1038/s41598-017-11132-0.
Recent development and advancement of next-generation sequencing (NGS) technologies have enabled the determination of mitochondrial genome (mitogenome) at extremely efficiency. In this study, complete or partial mitogenomes for 19 cicadomorphan species and six fulgoroid species were reconstructed by using the method of high-throughput sequencing from pooled DNA samples. Annotation analyses showed that the mitogenomes obtained have the typical insect mitogenomic content and structure. Combined with the existing hemipteran mitogenomes, a series of datasets with all 37 mitochondrial genes (up to 14,381 nt total) under different coding schemes were compiled to test previous hypotheses of deep-level phylogeny of Cicadomorpha. Thirty-seven species representing Cicadomorpha constituted the ingroup. A taxon sampling with nine species from Fulgoroidea and six from Heteroptera comprised the outgroup. The phylogenetic reconstructions congruently recovered the monophyly of each superfamily within Cicadomorpha. Furthermore, the hypothesis (Membracoidea + (Cicadoidea + Cercopoidea)) was strongly supported under the heterogeneous CAT model.
近年来，下一代测序（NGS）技术的发展和进步使得极其高效地测定线粒体基因组（mitogenome）成为可能。在这项研究中，通过对混合 DNA 样本进行高通量测序的方法，重建了 19 种蝉形目物种和 6 种叶蝉科物种的完整或部分线粒体基因组。注释分析表明，获得的线粒体基因组具有典型的昆虫线粒体基因组的内容和结构。结合现有的半翅目线粒体基因组，编译了一系列具有不同编码方案的 37 个线粒体基因（总共 14381nt）的数据集，以检验蝉形目深层级系统发育的先前假设。37 个代表蝉形目的物种构成了内群。外群由 9 种叶蝉科和 6 种半翅目物种组成。系统发育重建一致地恢复了蝉形目内每个超科的单系性。此外，在异质 CAT 模型下，（Membracoidea+（Cicadoidea+Cercopoidea））假设得到了强有力的支持。
