Liu Yan, Liang Jing, Zhang Zenghu, Yu Min, Wang Min, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2015 Nov;65(11):3950-3958. doi: 10.1099/ijsem.0.000518.
A Gram-staining-positive, strictly aerobic, spore-forming and rod-shaped motile bacterium with peritrichous flagellae, designated strain S1203T, was isolated from the sediment of the northern Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1203T formed a lineage within the family Bacillaceae that was distinct from the most closely related genera Bacillus, Bhargavaea, Planomicrobium and Virgibacillus with gene sequence similarities ranging from 86.2 to 93.76 %. Optimal growth occurred in the presence of 4-8 % (w/v) NaCl, at pH 7.0-8.0 and 25-32 °C. The cell-wall peptidoglycan was based on meso-diaminopimelic acid and unsaturated menaquinone with seven isoprene units (MK-7) as the predominant respiratory quinone. The major fatty acids (>10 % of total fatty acids) were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. The DNA G+C content of strain S1203T was 47.7 mol%. On the basis of polyphasic analysis, strainS1203T was considered to represent a novel species in a new genus of the family Bacillaceae, for which the name Aureibacillus halotolerans gen. nov., sp. nov. is proposed; the type strain of Aureibacillus halotolerans is S1203T ( = DSM 28697T = JCM 30067T = MCCC 1K00259T).
从冲绳海槽北部沉积物中分离出一株革兰氏染色阳性、严格需氧、产芽孢、杆状且具周生鞭毛的运动细菌，命名为菌株S1203T。基于16S rRNA基因序列的系统发育分析表明，菌株S1203T在芽孢杆菌科内形成了一个分支，与最相近的芽孢杆菌属、巴尔加瓦菌属、扁平微杆菌属和嗜盐芽孢杆菌属不同，基因序列相似性在86.2%至93.76%之间。在4-8%（w/v）NaCl、pH 7.0-8.0和25-32°C条件下生长最佳。细胞壁肽聚糖基于内消旋二氨基庚二酸，主要呼吸醌为含7个异戊二烯单元的不饱和甲基萘醌（MK-7）。主要脂肪酸（占总脂肪酸的>10%）为anteiso-C15:0、iso-C15:0和C16:0。主要极性脂为磷脂酰甘油、双磷脂酰甘油、一种未鉴定的糖脂和一种未鉴定的磷脂。菌株S1203T的DNA G+C含量为47.7mol%。基于多相分析，菌株S1203T被认为代表芽孢杆菌科一个新属中的一个新物种，为此提出新属名嗜盐金色芽孢杆菌属（Aureibacillus），新种名嗜盐嗜盐金色芽孢杆菌（Aureibacillus halotolerans）；嗜盐嗜盐金色芽孢杆菌的模式菌株为S1203T（=DSM 28697T =JCM 30067T =MCCC 1K00259T）。
