Department of Biological Sciences, Faculty of Science, Kuwait University, Safat 13060, Kuwait.
Department of Earth, Ocean and Atmospheric Sciences, Faculty of Science, The University of British Columbia, Vancouver, Canada.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004792.
A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1 was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf. Strain SIJ1 grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl. The cell-wall peptidoglycan was based on -diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone. It contained anteiso-C, iso-C and iso-C as the major fatty acids and ribose as the major whole-cell sugar. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid. Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1 showed a distinct evolutionary lineage within the Firmicutes. The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1 showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes. The SIJ1 annotated genome showed more stress resistance encoding genes in comparison to its closely related strains. The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1 does indeed represent a novel genus. The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties. On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1 represents a novel genus and species in the family Bacillaceae, for which the name gen. nov., sp. nov. is proposed. The type strain is SIJ1 (=DSM 28862=LMG 28316).
一株革兰氏阳性、严格需氧、产芽孢、杆状且不运动的细菌，被命名为 SIJ1 菌株，从科威特湾北岸的潮滩沉积物中分离得到，位于阿拉伯湾西北部。SIJ1 菌株在 30°C 和 pH7-8 的条件下，在 6%（w/v）NaCl 的存在下生长最佳。细胞壁肽聚糖基于 -二氨基庚二酸，并且主要的呼吸醌是具有七个异戊二烯单元的 MK-7（menaquinone）。它含有 anteiso-C、iso-C 和 iso-C 作为主要脂肪酸，以及核糖作为主要的全细胞糖。极性脂包括二磷脂酰甘油、磷脂酰甘油、未鉴定的磷脂、未鉴定的糖脂、磷酸甘油酯和未鉴定的脂。基于 16S rRNA 基因的系统发育分析表明，SIJ1 在厚壁菌门中表现出独特的进化谱系。DNA G+C 含量为 43.1mol%，对 SIJ1 菌株的全基因组分析表明，其基因组大小为 3,989,945bp，包含 4085 个预测的蛋白编码基因。SIJ1 菌株注释基因组与密切相关的菌株相比，具有更多的抗应激编码基因。全基因组的氨基酸身份和平均核苷酸身份数据证明，菌株 SIJ1 确实代表了一个新属。该菌株通过几个表型特性与系统发育上相关的属区分开来。基于表型、系统发育和遗传数据，SIJ1 菌株代表了芽孢杆菌科的一个新属和新种，建议采用新属名 gen. nov.，新种名 sp. nov.。模式菌株为 SIJ1（=DSM 28862=LMG 28316）。
