• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过神经元树突和棘突中的再循环内体的高尔基体非依赖性分泌运输。

Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines.

机构信息

Department of Pharmacology, University of Colorado School of Medicine, Aurora, United States.

Center for Psychiatry and Neurosciences, University Paris-Descartes, Paris, France.

出版信息

Elife. 2017 Sep 6;6:e27362. doi: 10.7554/eLife.27362.

DOI:10.7554/eLife.27362
PMID:28875935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5624785/
Abstract

Neurons face the challenge of regulating the abundance, distribution and repertoire of integral membrane proteins within their immense, architecturally complex dendritic arbors. While the endoplasmic reticulum (ER) supports dendritic translation, most dendrites lack the Golgi apparatus (GA), an essential organelle for conventional secretory trafficking. Thus, whether secretory cargo is locally trafficked in dendrites through a non-canonical pathway remains a fundamental question. Here we define the dendritic trafficking itinerary for key synaptic molecules in rat cortical neurons. Following ER exit, the AMPA-type glutamate receptor GluA1 and neuroligin 1 undergo spatially restricted entry into the dendritic secretory pathway and accumulate in recycling endosomes (REs) located in dendrites and spines before reaching the plasma membrane. Surprisingly, GluA1 surface delivery occurred even when GA function was disrupted. Thus, in addition to their canonical role in protein recycling, REs also mediate forward secretory trafficking in neuronal dendrites and spines through a specialized GA-independent trafficking network.

摘要

神经元面临着在其巨大、结构复杂的树突状分支中调节整合膜蛋白的丰度、分布和库的挑战。虽然内质网 (ER) 支持树突状翻译，但大多数树突缺乏高尔基体 (GA)，这是传统分泌运输的必需细胞器。因此，分泌货物是否通过非经典途径在树突中局部运输仍然是一个基本问题。在这里，我们定义了大鼠皮质神经元中关键突触分子的树突状运输途径。在 ER 出口后，AMPA 型谷氨酸受体 GluA1 和神经连接蛋白 1 经历空间受限进入树突状分泌途径，并在到达质膜之前积累在位于树突和棘突中的再循环内体 (RE) 中。令人惊讶的是，即使 GA 功能受到破坏，GluA1 表面的传递也会发生。因此，除了它们在蛋白质回收中的经典作用外，RE 还通过专门的 GA 独立的运输网络介导神经元树突和棘突中的正向分泌运输。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a8135cb8c26a/elife-27362-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/33812d827293/elife-27362-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/74b6f5d5c3e5/elife-27362-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/591c134d6f86/elife-27362-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/50388b6b345b/elife-27362-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/e9a97b3ee5b2/elife-27362-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3e3220e97e8d/elife-27362-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/941b796a6449/elife-27362-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a658282c8655/elife-27362-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/0b4bca94e599/elife-27362-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3ef592d49120/elife-27362-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3ee93318c208/elife-27362-fig4-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/25291de39a9e/elife-27362-fig4-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/8354e669f325/elife-27362-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3bfe5e9ac31b/elife-27362-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a046549d6f96/elife-27362-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/8fd174406c3d/elife-27362-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/d21908be242f/elife-27362-fig6-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/c414556bdb11/elife-27362-fig6-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a8135cb8c26a/elife-27362-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/33812d827293/elife-27362-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/74b6f5d5c3e5/elife-27362-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/591c134d6f86/elife-27362-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/50388b6b345b/elife-27362-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/e9a97b3ee5b2/elife-27362-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3e3220e97e8d/elife-27362-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/941b796a6449/elife-27362-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a658282c8655/elife-27362-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/0b4bca94e599/elife-27362-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3ef592d49120/elife-27362-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3ee93318c208/elife-27362-fig4-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/25291de39a9e/elife-27362-fig4-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/8354e669f325/elife-27362-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/3bfe5e9ac31b/elife-27362-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a046549d6f96/elife-27362-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/8fd174406c3d/elife-27362-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/d21908be242f/elife-27362-fig6-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/c414556bdb11/elife-27362-fig6-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac20/5624785/a8135cb8c26a/elife-27362-fig8.jpg

相似文献

1
Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines.通过神经元树突和棘突中的再循环内体的高尔基体非依赖性分泌运输。
Elife. 2017 Sep 6;6:e27362. doi: 10.7554/eLife.27362.
2
L-Type Voltage-Gated Ca Channels Regulate Synaptic-Activity-Triggered Recycling Endosome Fusion in Neuronal Dendrites.L 型电压门控钙通道调节神经元树突中突触活动触发的再循环内体融合。
Cell Rep. 2017 Nov 21;21(8):2134-2146. doi: 10.1016/j.celrep.2017.10.105.
3
Local zones of endoplasmic reticulum complexity confine cargo in neuronal dendrites.局部内质网复杂度区域限制神经元树突中的货物运输。
Cell. 2012 Jan 20;148(1-2):309-21. doi: 10.1016/j.cell.2011.11.056.
4
zapERtrap: A light-regulated ER release system reveals unexpected neuronal trafficking pathways.zapERtrap：一种光调控的内质网释放系统揭示了出人意料的神经元运输途径。
J Cell Biol. 2021 Sep 6;220(9). doi: 10.1083/jcb.202103186. Epub 2021 Jul 9.
5
Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity.肌球蛋白Vb动员回收型内体和AMPA受体以实现突触后可塑性。
Cell. 2008 Oct 31;135(3):535-48. doi: 10.1016/j.cell.2008.09.057.
6
Architecture and Dynamics of the Neuronal Secretory Network.神经元分泌网络的结构与动力学。
Annu Rev Cell Dev Biol. 2019 Oct 6;35:543-566. doi: 10.1146/annurev-cellbio-100818-125418. Epub 2019 Jul 5.
7
Functional compartmentalization of endosomal trafficking for the synaptic delivery of AMPA receptors during long-term potentiation.长时程增强过程中，内吞转运的功能区室化用于AMPA受体的突触传递。
J Neurosci. 2007 Nov 28;27(48):13311-5. doi: 10.1523/JNEUROSCI.4258-07.2007.
8
Dual modes of endoplasmic reticulum-to-Golgi transport in dendrites revealed by live-cell imaging.活细胞成像揭示树突内质网到高尔基体转运的双重模式
J Neurosci. 2003 Jul 16;23(15):6188-99. doi: 10.1523/JNEUROSCI.23-15-06188.2003.
9
Specialization of biosynthetic membrane trafficking for neuronal form and function.生物合成膜运输的特化对于神经元的形态和功能。
Curr Opin Neurobiol. 2016 Aug;39:8-16. doi: 10.1016/j.conb.2016.03.004. Epub 2016 Mar 22.
10
TRP Channel Trafficking瞬时受体电位通道转运

引用本文的文献

1
An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution.小鼠海马体在突触分辨率下的综合转录组学和蛋白质组学图谱。
Nat Commun. 2025 Aug 26;16(1):7942. doi: 10.1038/s41467-025-63119-5.
2
Endosomal protein DENND10 promotes developmental competence of neurite extension.内涵体蛋白DENND10促进神经突延伸的发育能力。
iScience. 2025 Apr 8;28(5):112385. doi: 10.1016/j.isci.2025.112385. eCollection 2025 May 16.
3
The impact of subunit type, alternative splicing, and auxiliary proteins on AMPA receptor trafficking.

本文引用的文献

1
Dendritic trafficking faces physiologically critical speed-precision tradeoffs.树突运输面临生理上至关重要的速度-精度权衡。
Elife. 2016 Dec 30;5:e20556. doi: 10.7554/eLife.20556.
2
Unconventional secretory processing diversifies neuronal ion channel properties.非常规分泌加工使神经元离子通道特性多样化。
Elife. 2016 Sep 28;5:e20609. doi: 10.7554/eLife.20609.
3
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope.使用定制的双色受激发射损耗显微镜对分离的嗅觉感觉神经元中的纤毛微域进行超分辨率成像。
亚基类型、可变剪接及辅助蛋白对AMPA受体转运的影响
J Biol Chem. 2025 Apr 30;301(6):108569. doi: 10.1016/j.jbc.2025.108569.
4
The neuronal Golgi in neural circuit formation and reorganization.神经回路形成与重组中的神经元高尔基体。
Front Neural Circuits. 2024 Dec 5;18:1504422. doi: 10.3389/fncir.2024.1504422. eCollection 2024.
5
Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.基因编码的内体探针用于标记和操纵 AMPA 型谷氨酸受体。
Nat Commun. 2024 Nov 29;15(1):10374. doi: 10.1038/s41467-024-54530-5.
6
The restricted N-glycome of neurons is programmed during differentiation.神经元受限的N-聚糖组在分化过程中被编程。
bioRxiv. 2024 Oct 17:2024.10.15.618477. doi: 10.1101/2024.10.15.618477.
7
Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.树突干中诱导的塑性肌动蛋白聚合调节细胞内 AMPA 受体运输。
Elife. 2024 Aug 15;13:e80622. doi: 10.7554/eLife.80622.
8
A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion.内质网高尔基复合体中间膜蛋白 10 介导的非典型蛋白分泌过程中内质网高尔基复合体定位 Rab 蛋白的双重作用
Nat Cell Biol. 2024 Jul;26(7):1077-1092. doi: 10.1038/s41556-024-01445-4. Epub 2024 Jun 26.
9
Rapid sequential clustering of NMDARs, CaMKII, and AMPARs upon activation of NMDARs at developing synapses.在发育中的突触处，NMDARs激活后，NMDARs、CaMKII和AMPARs迅速进行顺序性聚集。
Front Synaptic Neurosci. 2024 Apr 10;16:1291262. doi: 10.3389/fnsyn.2024.1291262. eCollection 2024.
10
A voyage from the ER: spatiotemporal insights into polarized protein secretion in neurons.从内质网出发的旅程：对神经元中极化蛋白分泌的时空洞察
Front Cell Dev Biol. 2023 Dec 22;11:1333738. doi: 10.3389/fcell.2023.1333738. eCollection 2023.
J Biomed Opt. 2016 Jun 1;21(6):66017. doi: 10.1117/1.JBO.21.6.066017.
4
A Dendritic Golgi Satellite between ERGIC and Retromer.内质网-高尔基体中间池与逆行运输蛋白复合物之间的树突状高尔基体卫星。
Cell Rep. 2016 Jan 12;14(2):189-99. doi: 10.1016/j.celrep.2015.12.024. Epub 2015 Dec 31.
5
Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture.AMPA 受体包含内体的定位调节突触结构。
Cell Rep. 2015 Nov 3;13(5):933-43. doi: 10.1016/j.celrep.2015.09.062. Epub 2015 Oct 22.
6
A general method to improve fluorophores for live-cell and single-molecule microscopy.一种改进用于活细胞和单分子显微镜的荧光团的通用方法。
Nat Methods. 2015 Mar;12(3):244-50, 3 p following 250. doi: 10.1038/nmeth.3256. Epub 2015 Jan 19.
7
Synaptopodin regulates spine plasticity: mediation by calcium stores.突触足蛋白调节脊柱可塑性：钙库介导。
J Neurosci. 2014 Aug 27;34(35):11641-51. doi: 10.1523/JNEUROSCI.0381-14.2014.
8
Synaptic control of secretory trafficking in dendrites.树突中分泌性运输的突触控制
Cell Rep. 2014 Jun 26;7(6):1771-8. doi: 10.1016/j.celrep.2014.05.028. Epub 2014 Jun 12.
9
Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABAB receptors.沿树突内质网的运输介导了GABAB受体的转运。
J Cell Sci. 2014 Aug 1;127(Pt 15):3382-95. doi: 10.1242/jcs.151092. Epub 2014 Jun 3.
10
Four-dimensional live imaging of apical biosynthetic trafficking reveals a post-Golgi sorting role of apical endosomal intermediates.顶部分泌性生物合成的四维活细胞成像揭示了高尔基体后阶段顶内体中间产物的分拣作用。
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4127-32. doi: 10.1073/pnas.1304168111. Epub 2014 Mar 3.