Epasinghe Don Jeevanie, Yiu Cynthia Kar Yung, Burrow Michael Francis
Prince Philip Dental Hospital, University of Hong Kong, Faculty of Dentistry, Hong Kong SAR, China.
University of Melbourne, Melbourne Dental School, Melbourne, Australia.
J Appl Oral Sci. 2017 Jul-Aug;25(4):412-419. doi: 10.1590/1678-7757-2016-0448.
This study evaluated the effect of grape seed extract (GSE) incorporation on the mechanical properties, water sorption, solubility, and GSE release from the experimental adhesive resins.
An experimental comonomer mixture, consisting of 40% Bis-GMA, 30% Bis MP, 28% HEMA, 0.26% camphorquinone and 1% EDMAB, was used to prepare four GSE-incorporated adhesive resins at concentrations of 0.5, 1, 1.5, and 2 wt%. The neat resin without GSE was used as the control. Six resin beams (25 mm x 2 mm x 2 mm) per group were prepared for flexural strength and modulus of elasticity evaluations using a universal testing machine at a crosshead speed of 1 mm/min. Five disks (6 mm in diameter and 2 mm in thickness) per group were used for microhardness measurements using a Leitz micro-hardness tester with Leica Qgo software. Five disks (7 mm in diameter and 2 mm in thickness) per group were prepared and stored in deionized water for 28 days. Water sorption, solubility, and GSE release in deionized water were calculated for each GSE-incorporated adhesive at the end of 28th day. Data was evaluated using one-way ANOVA and Tukey multiple comparisons.
Flexural strength, modulus of elasticity and microhardness of GSE-incorporated adhesive decreased significantly with incorporation of 1.5% of GSE (p<0.05). Addition of GSE had no effect on the water sorption of the adhesive resins (p=0.33). The solubility of the resin also increased significantly with incorporation of 1.5% of GSE (p<0.05). Quantities of GSE release increased with increased concentration of GSE in the adhesive resin.
Up to 1% of GSE can be incorporated into a dental adhesive resin without interfering with the mechanical properties or solubility of the resins.
本研究评估了添加葡萄籽提取物（GSE）对实验性粘结树脂的机械性能、吸水性、溶解性及GSE释放的影响。
使用一种由40%双酚A双甲基丙烯酸缩水甘油酯（Bis-GMA）、30%双酚甲基丙烯酸缩水甘油酯（Bis MP）、28%甲基丙烯酸羟乙酯（HEMA）、0.26%樟脑醌和1%乙胺丁醇（EDMAB）组成的实验性共聚单体混合物，制备了四种添加不同浓度GSE的粘结树脂，GSE浓度分别为0.5%、1%、1.5%和2%（质量分数）。未添加GSE的纯树脂用作对照。每组制备六个树脂梁（25 mm×2 mm×2 mm），使用万能试验机以1 mm/min的十字头速度进行弯曲强度和弹性模量评估。每组使用五个圆盘（直径6 mm，厚度2 mm），通过带有徕卡Qgo软件的徕卡显微硬度计进行显微硬度测量。每组制备五个圆盘（直径7 mm，厚度2 mm），并在去离子水中储存28天。在第28天结束时，计算每种添加GSE的粘结剂在去离子水中的吸水性、溶解性和GSE释放量。数据采用单因素方差分析和Tukey多重比较进行评估。
添加1.5% GSE后，添加GSE的粘结剂的弯曲强度、弹性模量和显微硬度显著降低（p<0.05）。添加GSE对粘结树脂的吸水性没有影响（p=0.33）。添加1.5% GSE后，树脂的溶解性也显著增加（p<0.05）。粘结树脂中GSE的释放量随GSE浓度的增加而增加。
在牙科粘结树脂中添加高达1%的GSE不会影响树脂的机械性能或溶解性。
