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酚类化合物对酰基高丝氨酸内酯介导的群体感应调控途径的影响。

Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways.

机构信息

Laboratory of Clinical Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.

Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA), 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, 39660, Republic of Korea.

出版信息

Sci Rep. 2017 Sep 6;7(1):10618. doi: 10.1038/s41598-017-10997-5.

DOI:10.1038/s41598-017-10997-5
PMID:28878346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5587592/
Abstract

Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa, and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compounds. Only methyl gallate (MG) among these compounds demonstrated profound anti-QS effect in the preliminary study, and thus only MG was utilized further to evaluate the effects on the synthesis and activity of acyl homoserine lactone (AHL) in C. violaceum and on the modulation of biofilm, motility, proteolytic, elastase, pyocyanin, and rhamnolipid activity in P. aeruginosa. Finally, the effect of MG on the expression of QS-regulated genes of P. aeruginosa was verified. MG suppressed both the synthesis and activity of AHL in C. violaceum. It also restricted the biofilm formation and other QS-associated virulence factor of P. aeruginosa. MG concentration-dependently suppressed the expression of lasI/R, rhlI/R, and pqsA of P. aeruginosa and was non-toxic in in vitro study. This is the first report of the anti-QS mechanism of MG.

摘要

群体感应 (QS) 是一种由自动诱导物介导的、依赖于细胞密度的毒力细菌基因表达调控，可能与细菌毒力的流行有关。本研究最初旨在评估 5 种酚类化合物对粘质沙雷氏菌和铜绿假单胞菌的 QS 和毒力因子的调节作用，并确定其作用机制。生物传感器菌株被用于评估这些化合物的抗菌和抗 QS 作用。在初步研究中，只有没食子酸甲酯 (MG) 表现出明显的抗 QS 作用，因此仅使用 MG 进一步评估其对粘质沙雷氏菌中酰基高丝氨酸内酯 (AHL) 的合成和活性以及对生物膜、运动性、蛋白水解酶、弹性蛋白酶、绿脓菌素和鼠李糖脂活性的调节作用。最后，验证了 MG 对铜绿假单胞菌中 QS 调控基因表达的影响。MG 抑制了粘质沙雷氏菌中 AHL 的合成和活性。它还限制了铜绿假单胞菌生物膜的形成和其他与 QS 相关的毒力因子。MG 浓度依赖性地抑制了铜绿假单胞菌的 lasI/R、rhlI/R 和 pqsA 的表达，并且在体外研究中无毒性。这是 MG 抗 QS 机制的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/ea6d15fc1934/41598_2017_10997_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/9cce93530b91/41598_2017_10997_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/74a0f6e0361d/41598_2017_10997_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/91e1a3063e09/41598_2017_10997_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/5f8b2b2aac02/41598_2017_10997_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/94ff04568dbf/41598_2017_10997_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/4e107f1c8b8f/41598_2017_10997_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/3b6e5007d112/41598_2017_10997_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/4e74cf9b81fd/41598_2017_10997_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/ea6d15fc1934/41598_2017_10997_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/9cce93530b91/41598_2017_10997_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/74a0f6e0361d/41598_2017_10997_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/91e1a3063e09/41598_2017_10997_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/5f8b2b2aac02/41598_2017_10997_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/94ff04568dbf/41598_2017_10997_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/4e107f1c8b8f/41598_2017_10997_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/3b6e5007d112/41598_2017_10997_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/4e74cf9b81fd/41598_2017_10997_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fa/5587592/ea6d15fc1934/41598_2017_10997_Fig9_HTML.jpg

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