Duan Guman, Li Chengli, Yan Xiaoqing, Yang Shuhui, Wang Shuo, Sun Xiaodan, Zhao Lingyun, Song Tianxi, Pan Yongwei, Wang Xiumei
State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
Department of Orthopedics, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102218, China.
Regen Biomater. 2022 Oct 31;10:rbac089. doi: 10.1093/rb/rbac089. eCollection 2023.
A new nerve guidance conduits (NGCs) named MC@Col containing Type I collagen (Col) and mineralized collagen (MC) was developed, enhancing mechanical and degradation behavior. The physicochemical properties, the mechanical properties and degradation behavior were all evaluated. The adhesion and proliferation of Schwann cells (SCs) were observed. In the experiment, MC@Col NGC and other conduits including Col, chitosan (CST) and polycaprolactone (PCL) conduit were implanted to repair a 10-mm-long Sprague-Dawley rat's sciatic nerve defect. Histological analyses, morphological analyses, electrophysiological analyses and further gait analyses were all evaluated after implantation in 12 weeks. The strength and degradation performance of the MC@Col NGC were improved by the addition of MC in comparison with pure Col NGC. cytocompatibility evaluation revealed that the SCs had good viability, attachment and proliferation in the MC@Col. In results, the regenerative outcomes of MC@Col NGC were close to those by an autologous nerve graft in some respects, but superior to those by Col, CST and PCL conduits. The MC@Col NGC exhibited good mechanical performance as well as biocompatibility to bridge nerve gap and guide nerve regeneration, thus showing great promising potential as a new type of conduit in clinical applications.
研发出一种名为MC@Col的新型神经引导导管（NGC），其含有I型胶原蛋白（Col）和矿化胶原蛋白（MC），可增强机械性能和降解行为。对其物理化学性质、机械性能和降解行为进行了评估。观察了雪旺细胞（SCs）的黏附与增殖情况。在实验中，植入MC@Col NGC以及其他导管，包括Col、壳聚糖（CST）和聚己内酯（PCL）导管，以修复10毫米长的Sprague-Dawley大鼠坐骨神经缺损。植入12周后，对其进行组织学分析、形态学分析、电生理分析以及进一步的步态分析。与纯Col NGC相比，添加MC提高了MC@Col NGC的强度和降解性能。细胞相容性评估显示，SCs在MC@Col中具有良好的活力、附着和增殖能力。结果表明，MC@Col NGC的再生效果在某些方面接近自体神经移植，但优于Col、CST和PCL导管。MC@Col NGC表现出良好的机械性能以及生物相容性，可桥接神经间隙并引导神经再生，因此作为一种新型导管在临床应用中显示出巨大的潜力。
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