Nawrotek Katarzyna, Tylman Michał, Rudnicka Karolina, Gatkowska Justyna, Balcerzak Jacek
Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland.
Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland.
J Mech Behav Biomed Mater. 2016 Jul;60:256-266. doi: 10.1016/j.jmbbm.2016.02.012. Epub 2016 Feb 11.
A new approach for obtaining chitosan-carbon nanotube implants enriched with calcium ions in the form of tubular hydrogels is fostered. The intended application of the hydrogels is tissue engineering, especially peripheral nervous tissue regeneration. The fabrication method, based on an electrodeposition phenomenon, shows significant advantages over current solutions as implants can now be obtained rapidly at any required dimensions. Thus, it may open a new avenue to treat patients with peripheral nerve injuries. Either single walled or multiwalled carbon nanotubes enhance the mechanical properties of the tubular hydrogels. The controlled presence of calcium ions, sourced from hydroxyapatite, is also expected to augment the regenerative response. Because in vitro cytotoxic assays on mouse cell lines (L929 fibroblasts and mHippoE-18 hippocampal cells) as well as pro-inflammatory tests on THP-1XBlue™ cells show that the manufactured implants are biocompatible, we next intend to evaluate their immune- and nervous-safety on an animal model.
一种制备富含钙离子的壳聚糖-碳纳米管植入物的新方法正在兴起，该植入物呈管状水凝胶形式。这些水凝胶的预期应用是组织工程，特别是周围神经组织再生。基于电沉积现象的制造方法显示出相对于现有解决方案的显著优势，因为现在可以快速获得任何所需尺寸的植入物。因此，它可能为治疗周围神经损伤患者开辟一条新途径。单壁或多壁碳纳米管均可增强管状水凝胶的机械性能。来自羟基磷灰石的钙离子的可控存在也有望增强再生反应。由于对小鼠细胞系（L929成纤维细胞和mHippoE-18海马细胞）的体外细胞毒性测定以及对THP-1XBlue™细胞的促炎测试表明，所制造的植入物具有生物相容性，我们接下来打算在动物模型上评估它们的免疫和神经安全性。
