高效液相色谱-蒸发光散射检测法测定不同产地醉鱼草果实中3种三萜皂苷的含量

[HPLC-ELSD for determining contents of three triterpenoidal saponins in fruits of Buddleja lindleyana from different habitats].

作者信息

Wu Pei-Yun, Ren Ya-Shuo, Wu De-Ling, Qiao Jin-Wei

机构信息

School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.

Anhui Key Laboratory of Modernized Chinese Materia Medica, Hefei 230012, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2016 Apr;41(7):1218-1221. doi: 10.4268/cjcmm20160710.

DOI:10.4268/cjcmm20160710

PMID:28879734

Abstract

To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RP-HPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits, which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters SunFireTM C₁₈ (4.6 mm×150 mm, 5 μm). The mobile phase consisted of Methanol-water(82∶18) at a flow rate of 1 mL•min⁻¹. Column temperature： 30 ℃. ELSD conditions： drift tube temperature： 106 ℃; carrier gas (nitrogen) flow rate： 1.5 L•min⁻¹; amplification factor： 1. The calibration curves showed good linear relationship on a range from 0.702 to 28.08 μg(r=0.999 2) for Clinoposaponin III, 0.390 to 15.60 μg(r=0.998 9) for Desrhamnoverbascosaponin and 0.192 to 7.68μg(r=0.999 0) for Mimengoside I. The average recovery rate(n=6) were 99.41%, 99.08% and 98.67% and it's RSD were 0.86%, 1.56% and 1.80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity, accurateness and reliability.

摘要

建立一种用于定量测定醉鱼草果实中三萜类化合物的高效液相色谱 - 蒸发光散射检测法（HPLC - ELSD）。采用反相高效液相色谱 - 蒸发光散射检测法测定采集自不同产地的醉鱼草果实中的三萜类化合物。所用色谱柱为填充有5μm固定相的沃特世SunFireTM C₁₈柱（4.6 mm×150 mm，5μm）。流动相为甲醇 - 水（82∶18），流速为1 mL•min⁻¹。柱温：30℃。蒸发光散射检测条件：漂移管温度：106℃；载气（氮气）流速：1.5 L•min⁻¹；放大因子：1。校准曲线表明，齐墩果酸 - 3 - O - 葡萄糖醛酸苷Ⅲ在0.702至28.08μg范围内线性关系良好（r = 0.999 2），去鼠李糖婆婆纳苷在0.390至15.60μg范围内线性关系良好（r = 0.998 9），迷蒙苷Ⅰ在0.192至7.68μg范围内线性关系良好（r = 0.999 0）。平均回收率（n = 6）分别为99.41%、99.08%和98.67%，相对标准偏差分别为0.86%、1.56%和1.80%。该方法简便、准确、可靠，可用于测定醉鱼草果实中三萜类化合物的含量。