Wang Jing-Zhe, Xu Feng, Liu Zhen, Ma Li-Man, Shang Ming-Ying, Liu Guang-Xue, Cai Shao-Qing
School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Zhongguo Zhong Yao Za Zhi. 2016 Apr;41(7):1257-1268. doi: 10.4268/cjcmm20160717.
This experiment was performed to establish a qualitative analysis on chemical constituents of Scrophulariae Radix by HPLC-ESI-IT-TOF-MS．The analysis was conducted on a C₁₈ column (Kromasil 100-5, 4.6 mm×250 mm, 5 μm) with 0.1% formic acid-acetonitrile as the mobile phase for gradient elution; ESI ion source was used for mass spectra, and data were collected innegative and positive modes. The results showed that 64 compounds from Scrophulariae Radix had been identified by analyzing negative ion mass data including element composition and by comparing with data from literature. Two new compounds (4-hydroxy-6-O-methylcatalpol and acetylangoroside C) and seventeen known compounds were detected from Scrophulariae Radix for the first time. Seventeen known compounds included twelve iridoid glycosides, three phenylpropanoid glycosides and two other kind compounds. This study will provide chemical basis for elucidation of the effective substance in the Scrophulariae Radix.
本实验旨在通过高效液相色谱-电喷雾电离-离子阱-飞行时间质谱（HPLC-ESI-IT-TOF-MS）对玄参的化学成分进行定性分析。分析在C₁₈柱（Kromasil 100-5，4.6 mm×250 mm，5μm）上进行，以0.1%甲酸-乙腈为流动相进行梯度洗脱；采用电喷雾电离（ESI）离子源进行质谱分析，数据以正、负离子模式采集。结果表明，通过分析负离子质量数据（包括元素组成）并与文献数据比较，已从玄参中鉴定出64种化合物。首次从玄参中检测到两种新化合物（4-羟基-6-O-甲基梓醇和乙酰安哥洛苷C）以及17种已知化合物。17种已知化合物包括12种环烯醚萜苷、3种苯丙素苷和其他两类化合物。本研究将为阐明玄参中的有效物质提供化学依据。
