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使用具有有机硅、葡聚糖和聚乙二醇涂层的超小超顺磁性氧化铁纳米颗粒对间充质干细胞进行直接标记的效果和耐久性

Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings.

作者信息

Wang Yi-Xiang J, Quercy-Jouvet Thibault, Wang Hao-Hao, Li Ak-Wai, Chak Chun-Pong, Xuan Shouhu, Shi Lin, Wang De-Feng, Lee Siu-Fung, Leung Ping-Chung, Lau Clara B S, Fung Kwok-Pui, Leung Ken Cham-Fai

机构信息

Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.

Center of Novel Functional Molecules and Institute of Molecular Functional Materials, Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.

出版信息

Materials (Basel). 2011 Apr 7;4(4):703-715. doi: 10.3390/ma4040703.

DOI:10.3390/ma4040703
PMID:28879947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5448517/
Abstract

We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6-7 nm. Together with their coatings, the overall sizes are 10-15 nm for all SPIO@SiO₂, SPIO@dextran, and SPIO@PEG nanoparticles. These nanoparticles were investigated for their efficacies to be uptaken by rabbit bone marrow-derived MSCs without any transfecting agent. Experimentally, both SPIO@SiO₂ and SPIO@PEG nanoparticles could be successfully uptaken by MSCs while the SPIO@dextran nanoparticles demonstrated limited labeling efficiency. The labeling durability of SPIO@SiO₂ and SPIO@PEG nanoparticles in MSCs after three weeks of culture were compared by Prussian blue staining tests. SPIO@SiO₂ nanoparticles demonstrated more blue staining than SPIO@PEG nanoparticles, rendering them better materials for MSCs labeling by direct uptake when durable intracellullar retention of SPIO is desired.

摘要

我们在此报告一项关于使用含有不同涂层（即有机硅、葡聚糖和聚乙二醇（PEG））的球形超顺磁性氧化铁（SPIO）纳米颗粒对间充质干细胞（MSC）进行标记的比较研究。这些纳米材料具有相似的6 - 7纳米的SPIO核心尺寸。连同它们的涂层，所有SPIO@SiO₂、SPIO@葡聚糖和SPIO@PEG纳米颗粒的总体尺寸为10 - 15纳米。在没有任何转染剂的情况下，研究了这些纳米颗粒被兔骨髓来源的间充质干细胞摄取的效率。实验表明，SPIO@SiO₂和SPIO@PEG纳米颗粒都能被间充质干细胞成功摄取，而SPIO@葡聚糖纳米颗粒的标记效率有限。通过普鲁士蓝染色试验比较了SPIO@SiO₂和SPIO@PEG纳米颗粒在间充质干细胞中培养三周后的标记耐久性。SPIO@SiO₂纳米颗粒显示出比SPIO@PEG纳米颗粒更多的蓝色染色，当需要SPIO在细胞内持久保留时，使其成为通过直接摄取对间充质干细胞进行标记的更好材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/7cf60d819936/materials-04-00703-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/d038bfe6f20a/materials-04-00703-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/059a5ef9bb09/materials-04-00703-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/7cf60d819936/materials-04-00703-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/d038bfe6f20a/materials-04-00703-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/059a5ef9bb09/materials-04-00703-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7f6/5448517/7cf60d819936/materials-04-00703-g003.jpg

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