Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
Nat Struct Mol Biol. 2017 Sep 7;24(9):689-699. doi: 10.1038/nsmb.3454.
Eukaryotic ribosome synthesis is a complex, energy-consuming process that takes place across the nucleolus, nucleoplasm and cytoplasm and requires more than 200 conserved assembly factors. Here, we discuss mechanisms by which the ribosome assembly and nucleocytoplasmic transport machineries collaborate to produce functional ribosomes. We also highlight recent cryo-EM studies that provided unprecedented snapshots of ribosomes during assembly and quality control.
真核生物核糖体合成是一个复杂的、耗能的过程，发生在核仁、核质和细胞质中，需要 200 多种保守的组装因子。在这里，我们讨论了核糖体组装和核质转运机制如何协作以产生功能性核糖体。我们还强调了最近的冷冻电镜研究，这些研究提供了在组装和质量控制过程中核糖体的前所未有的快照。
