Kressler Dieter, Hurt Ed, Bassler Jochen
Biochemie-Zentrum der Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
Biochim Biophys Acta. 2010 Jun;1803(6):673-83. doi: 10.1016/j.bbamcr.2009.10.009. Epub 2009 Oct 30.
Ribosome biogenesis is a fundamental process that provides cells with the molecular factories for cellular protein production. Accordingly, its misregulation lies at the heart of several hereditary diseases (e.g., Diamond-Blackfan anemia). The process of ribosome assembly comprises the processing and folding of the pre-rRNA and its concomitant assembly with the ribosomal proteins. Eukaryotic ribosome biogenesis relies on a large number (>200) of non-ribosomal factors, which confer directionality and accuracy to this process. Many of these non-ribosomal factors fall into different families of energy-consuming enzymes, notably including ATP-dependent RNA helicases, AAA-ATPases, GTPases, and kinases. Ribosome biogenesis is highly conserved within eukaryotic organisms; however, due to the combination of powerful genetic and biochemical methods, it is best studied in the yeast Saccharomyces cerevisiae. This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes.
核糖体生物合成是一个为细胞提供用于细胞蛋白质生产的分子工厂的基本过程。因此，其失调是几种遗传性疾病（如钻石黑范贫血症）的核心问题。核糖体组装过程包括前体rRNA的加工和折叠以及它与核糖体蛋白的伴随组装。真核生物的核糖体生物合成依赖于大量（>200个）非核糖体因子，这些因子赋予了这一过程方向性和准确性。这些非核糖体因子中的许多属于不同家族的耗能酶，特别是包括ATP依赖性RNA解旋酶、AAA-ATP酶、GTP酶和激酶。核糖体生物合成在真核生物中高度保守；然而，由于强大的遗传学和生物化学方法的结合，它在酿酒酵母中得到了最好的研究。本综述总结了我们目前对真核生物核糖体组装的认识，特别关注所涉及的耗能酶的分子作用。
