CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.
Academy of Scientific and Innovative Research (AcSIR-CSIO) , Chandigarh 160030, India.
ACS Appl Mater Interfaces. 2017 Oct 4;9(39):33589-33598. doi: 10.1021/acsami.7b07818. Epub 2017 Sep 21.
To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal-organic framework (MOF), NH-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF-bacteriophage biosensor based on the photoluminescence quenching phenomena: limit of detection (31 CFU/mL) and range of detection (40 to 4 × 10 CFU/mL). This is the first report exploiting the use of an MOF-bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity, regenerability, extended range of linear detection, and stability for long-term storage (even at room temperature).
为了生产一种对金黄色葡萄球菌敏感且特异的生物传感器，噬菌体已与一种水分散且环境稳定的金属有机骨架（MOF）NH-MIL-53（Fe）相连接。MOF 与噬菌体的连接是通过使用戊二醛作为交联剂来实现的。基于光致发光猝灭现象，所提出的 MOF-噬菌体生物传感器实现了对合成和实际样品中金黄色葡萄球菌的高灵敏度检测：检测限（31 CFU/mL）和检测范围（40 到 4 × 10 CFU/mL）。这是首次报道利用 MOF-噬菌体复合物来进行金黄色葡萄球菌的生物传感。我们的研究结果表明，与大多数先前的方法相比，所提出的生物传感器具有更高的灵敏度，同时还具有一些先进的特性，如特异性、可再生性、线性检测范围扩展以及长期储存稳定性（即使在室温下）。
