Cheng Wenming, Zhang Xun, Song Qiang, Lu Weili, Wu Tingni, Zhang Qunlin, Li Chunru
School of Pharmacy, Anhui Provincial Key Laboratory of Bioactivity of Natural Product, Anhui Medical University, Hefei, Anhui, China.
Zhejiang BioAsia Institute of Life Science, Pinghu, Zhejiang, China.
Mycology. 2017 Oct 26;8(4):318-326. doi: 10.1080/21501203.2017.1385546. eCollection 2017.
Nucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of (NFOS). Five nucleosides or nucleobases - adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) - were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of and its allies.
核苷和核碱基是质量控制的最重要指标之一。采用灵敏可靠的高效液相色谱 - 紫外检测法，同时分析中国9种及其近缘种15批次样品中的13种核苷和核碱基。运用SPSS 22.0软件（美国纽约州阿蒙克市IBM公司）进行主成分分析（PCA）和聚类分析。基于核苷和核碱基含量成功区分了15个样品。菌丝体中总核苷含量显著高于天然子实体（NFOS）。根据主成分分析，腺嘌呤（A）、鸟苷（Gu）、尿嘧啶（U）、尿苷（Ur）和鸟嘌呤（G）这5种核苷或核碱基是导致总变异的主要成分。所检测的13种核苷和核碱基（包括腺苷、胞苷、鸟苷、肌苷、胸苷、尿苷、虫草素、腺嘌呤、胞嘧啶、鸟嘌呤、胸腺嘧啶、尿嘧啶和次黄嘌呤）的图谱能够区分不同样品，可作为冬虫夏草及其近缘种质量控制的候选指标。
