聚糖刺激可实现基于 PEDOT 纳米魔术贴芯片的前列腺癌循环肿瘤细胞的纯化，从而用于 RNA 生物标志物检测。
Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection.
机构信息
Smart Organic Material Laboratory, Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nankang, Taipei, 11529, Taiwan.
Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, CA, 90048, USA.
出版信息
Adv Healthc Mater. 2018 Feb;7(3). doi: 10.1002/adhm.201700701. Epub 2017 Sep 11.
Abstract
A glycan-stimulated and poly(3,4-ethylene-dioxythiophene)s (PEDOT)-based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)-grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT-PBA-co-EDOT-EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well-preserved RNA transcripts for the analysis of the expression level of several PCa-specific RNA biomarkers, which may provide clinical insights into the disease.
摘要
一种糖刺激的和聚（3,4-亚乙基二氧噻吩）（PEDOT）基纳米材料平台被构建用于从前列腺癌（PCa）患者的血液样本中纯化循环肿瘤细胞（CTC）。这个新平台，苯硼酸（PBA）接枝的 PEDOT NanoVelcro，将 3D PEDOT 纳米基底与聚（EDOT-PBA-co-EDOT-EG3）界面层相结合，不仅在抗体偶联时提供了对 CTC 捕获的高特异性，而且还能够使山梨醇进行竞争性结合，从而温和地释放捕获的细胞。通过这种 PEDOT NanoVelcro 芯片纯化的 CTC 提供了保存完好的 RNA 转录本，可用于分析几种 PCa 特异性 RNA 生物标志物的表达水平，这可能为该疾病提供临床见解。
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