Das Sushant Swaroop, Mishra Sabita, Kaul J M
Senior Resident, Department of Anatomy, MAMC, New Delhi, India.
Director Professor, Department of Anatomy, MAMC, New Delhi, India.
J Clin Diagn Res. 2017 Jul;11(7):AC01-AC04. doi: 10.7860/JCDR/2017/26211.10225. Epub 2017 Jul 1.
The parafollicular cells or clear (C) cells in man are part of neuroendocrine system under Amine Precursor Uptake and Decarboxylation (APUD) cells. Their role in adults has been reputable but in foetus is still unclear.
The present study was a baseline study endeavouring to describe the chronological development of the parafollicular cells with particular focus on its correlation with developing human thyroid follicles.
The study was conducted on 10 aborted foetuses (14-28 weeks), procured from the Department of Obstetrics and Gynaecology, Lok Nayak Hospital, New Delhi, India. Serial sections of foetal thyroid gland were generated, stained with haematoxylin and eosin, and immunohistochemistry using the anticalcitonin antibody and examined qualitatively.
In our study, the parafollicular cells were seen as earlier as by 14 week. They became morphologically and functionally mature by 16 week of gestation. The parafollicular cells were getting organized from scattering to parafollicular location then to a more localized area, i.e., intrafollicular along with the follicular development. As the follicles were enlarging, the intrafollicularly located parafollicular cells which was initially present in groups was getting displaced singly between the follicular cells in the same follicle.
The sequential development pattern of the parafollicular cells in relation to developing thyroid follicles was established. This immunohistochemical study also concluded that the parafollicular cells might have higher character to play in the early gestational age such as regulation of ossification in the human foetus.
人类的滤泡旁细胞或透明（C）细胞是胺前体摄取和脱羧（APUD）细胞所属神经内分泌系统的一部分。它们在成年人中的作用已为人所知，但在胎儿中的作用仍不清楚。
本研究是一项基线研究，旨在描述滤泡旁细胞的时序发育，特别关注其与人类甲状腺滤泡发育的相关性。
本研究使用了从印度新德里洛卡·奈亚克医院妇产科获取的10例流产胎儿（14 - 28周）。制作胎儿甲状腺的连续切片，用苏木精和伊红染色，并使用抗降钙素抗体进行免疫组织化学染色，然后进行定性检查。
在我们的研究中，早在14周时就可见到滤泡旁细胞。它们在妊娠16周时在形态和功能上成熟。随着滤泡发育，滤泡旁细胞从散在分布逐渐组织化，先是位于滤泡旁，然后到更局限的区域，即滤泡内。随着滤泡增大，最初成组存在于滤泡内的滤泡旁细胞单个地被挤到同一滤泡的滤泡细胞之间。
确立了滤泡旁细胞与发育中的甲状腺滤泡相关的顺序发育模式。这项免疫组织化学研究还得出结论，滤泡旁细胞可能在妊娠早期发挥更重要的作用，比如对人类胎儿骨化的调节。
