Umamageswari M S, Karthikeyan T M, Maniyar Yasmeen A
Assistant Professor, Department of Pharmacology, Karpagam Faculty of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
Professor and Head, Department of Pathology, Karpagam Faculty of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
J Clin Diagn Res. 2017 Jul;11(7):FC16-FC19. doi: 10.7860/JCDR/2017/26563.10312. Epub 2017 Jul 1.
Linn. is a common medicinal plant possessing a wide variety of pharmacological activity. Current treatment of diabetes mellitus has plenty of adverse effects necessitating the search of alternate drugs.
Evaluation of antidiabetic effect of Aqueous Extract of Linn Berries (AESNB) in alloxan induced diabetic Wistar rats.
There were five groups (normal control, diabetic control, AESNB 200 mg/kg/day, ASENB 400 mg/kg/day and standard i.e., glimepride 0.1 mg/kg/day) with six animals in each group. Alloxan was used to induce diabetes in rats. The standard drug glimepride in the dose of 0.1 mg/kg/day and the test drug AESNB were given orally in the doses of 200 mg/kg/day and 400 mg/kg/day. The fasting blood glucose level was measured by glucometer on day 0, 1,7,14 and 21 after 12 hour. On 21 day after the blood glucose measurement all the animals were sacrificed and their pancreas were analysed histopathologically. The results were analysed statistically by using one-way ANOVA.
Aqueous extract of Linn berries in the dose of 200 mg/kg/day produced significant blood glucose reduction (p<0.01) from day 7 and 400 mg/kg/day produced highly significant reduction in blood glucose from day 7 (p<0.001). The standard drug glimepride reduced the blood glucose as equal to normal on day 21 (p<0.001). Histopathological examination showed the regeneration of pancreatic beta cells in AESNB group.
Aqueous extract of Linn berries possess antidiabetic activity.
Linn. 是一种常见的药用植物，具有多种药理活性。目前糖尿病的治疗存在诸多不良反应，因此需要寻找替代药物。
评估Linn浆果水提取物（AESNB）对四氧嘧啶诱导的糖尿病Wistar大鼠的抗糖尿病作用。
共分为五组（正常对照组、糖尿病对照组、AESNB 200毫克/千克/天组、ASENB 400毫克/千克/天组以及标准组，即格列美脲0.1毫克/千克/天组），每组六只动物。使用四氧嘧啶诱导大鼠患糖尿病。标准药物格列美脲剂量为0.1毫克/千克/天，受试药物AESNB剂量为200毫克/千克/天和400毫克/千克/天，均口服给药。在第0、1、7、14和21天禁食12小时后，用血糖仪测量空腹血糖水平。在测量血糖后的第21天，处死所有动物，并对其胰腺进行组织病理学分析。结果采用单因素方差分析进行统计学分析。
200毫克/千克/天剂量的Linn浆果水提取物从第7天起血糖显著降低（p<0.01），400毫克/千克/天剂量从第7天起血糖极显著降低（p<0.001）。标准药物格列美脲在第21天将血糖降至正常水平（p<0.001）。组织病理学检查显示AESNB组胰腺β细胞再生。
Linn浆果水提取物具有抗糖尿病活性。
