Xu Keheng, Zhao Yu, Zhao Yan, Feng Chen, Zhang Yinhe, Wang Fawei, Li Xiaowei, Gao Hongtao, Liu Weican, Jing Yan, Saxena Rachit K, Feng Xianzhong, Zhou Yonggang, Li Haiyan
College of Life Sciences, Jilin Agricultural University, Changchun, China.
College of Tropical Crops, Sanya Nanfan Research Institute, Hainan University, Haikou, China.
Front Plant Sci. 2022 Jun 2;13:823529. doi: 10.3389/fpls.2022.823529. eCollection 2022.
The gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In this study, bioinformatics analysis of the soybean gene family was performed, and the role of soybean gene in drought stress adaptation was characterized. We identified 507 genes in the soybean genome database, which were classified into 11 subfamilies. The expression profiles showed that was highly expressed in plant roots. Overexpression of increased the sensitivity of transgenic to drought stress. Under drought stress, the hydrogen peroxide (HO) and malonaldehyde (MDA) contents of transgenic were higher than those of the wild type (WT) and empty vector control, and the chlorophyll content was lower than that of the control. Y2H and bimolecular fluorescence complementation (BiFC) assays showed that GmFBL144 can interact with GmsHSP. Furthermore, our results showed that GmFBL144 can form SCF (E3 ubiquitin ligase) with GmSkp1 and GmCullin1. Altogether, these results indicate that the soybean F-box-like protein GmFBL144 may negatively regulate plant drought stress tolerance by interacting with sHSP. These findings provide a basis for molecular genetics and breeding of soybean.
该基因家族是植物中最大的基因家族之一。这些基因调控植物的生长发育以及生物和非生物胁迫反应，并且已经得到了广泛研究。干旱胁迫是限制大豆产量和品质的主要因素之一。在本研究中，对大豆该基因家族进行了生物信息学分析，并对大豆基因在干旱胁迫适应中的作用进行了表征。我们在大豆基因组数据库中鉴定出507个该基因，它们被分为11个亚家族。表达谱显示该基因在植物根中高度表达。该基因的过表达增加了转基因植株对干旱胁迫的敏感性。在干旱胁迫下，转基因植株的过氧化氢（HO）和丙二醛（MDA）含量高于野生型（WT）和空载体对照，叶绿素含量低于对照。酵母双杂交（Y2H）和双分子荧光互补（BiFC）分析表明，GmFBL144能与GmsHSP相互作用。此外，我们的结果表明，GmFBL144能与GmSkp1和GmCullin1形成SCF（E3泛素连接酶）。总之，这些结果表明，大豆类F-box蛋白GmFBL144可能通过与小分子热激蛋白（sHSP）相互作用来负调控植物的干旱胁迫耐受性。这些发现为大豆的分子遗传学和育种提供了依据。
