Laczkó-Zöld Eszter, Forgó Péter, Zupkó István, Sigrid Eşianu, Hohmann Judit
1 Department of Pharmacognosy, University of Medicine and Pharmacy Târgu Mureş , 540139 Târgu Mureş , Romania.
2 Department of Pharmacognosy, University of Szeged , H-6720 Szeged , Hungary.
Acta Biol Hung. 2017 Sep;68(3):300-309. doi: 10.1556/018.68.2017.3.7.
Physalin D was isolated from the methanol extract of Physalis alkekengi L. fruits by combination of different chromatographic methods (CPC, TLC, HPLC). The structure was elucidated based on H and C NMR spectral analysis with the aid of 2D-correlation spectroscopy (H, H-COSY, HSQC and HMBC) and comparison with literature data. The quantity of physalin D in mature and immature fruits and calyces was determined by RP-HPLC-UV method. Among the studied samples, immature calyx showed the highest content of physalin D (0.7880 ± 0.0612%), while mature calyx contained 4 times less amount (0.2028 ± 0.016%). The physalin D content of the fruit was much lower; immature fruits contained 0.0992 ± 0.0083% physalin D and mature fruits 0.0259 ± 0.0021%. The antiproliferative activity of the CHCl extract and its fractions was tested on three cancer cell lines (HeLa, MCF-7 and A431). The antiproliferative activity of physalin D is discussed with regard the published data.
酸浆苦素D是通过不同色谱方法（CPC、TLC、HPLC）相结合，从酸浆果实的甲醇提取物中分离得到的。借助二维相关光谱（H,H-COSY、HSQC和HMBC）并与文献数据比较，基于氢核磁共振和碳核磁共振光谱分析阐明了其结构。采用反相高效液相色谱-紫外检测法测定了成熟果实、未成熟果实及花萼中酸浆苦素D的含量。在所研究的样品中，未成熟花萼中酸浆苦素D的含量最高（0.7880±0.0612%），而成熟花萼中的含量则少4倍（0.2028±0.016%）。果实中酸浆苦素D的含量要低得多；未成熟果实中酸浆苦素D的含量为0.0992±0.0083%，成熟果实中为0.0259±0.0021%。对氯仿提取物及其馏分在三种癌细胞系（HeLa、MCF-7和A431）上进行了抗增殖活性测试。结合已发表的数据对酸浆苦素D的抗增殖活性进行了讨论。
