Zhang Hui, Chang Ya-Qi, Zheng Wei-Shuang, Chen Guan-Jun, Du Zong-Jun
State key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4083-4088. doi: 10.1099/ijsem.0.002257. Epub 2017 Sep 13.
A novel Gram-stain-negative, non-gliding, facultatively anaerobic and rod-shaped bacterium, designated X7, was isolated from marine sediment taken from the coast of Weihai, China. Strain X7 grew optimally at 28-30 °C, at pH 7.0 and in the presence of 2-3 % (w/v) NaCl. Based on the 16S rRNA gene sequence analysis, strain X7 was a member of the genus Salinimicrobium and was most closely related to the species Salinimicrobium gaetbulicola with a 96.3 % 16S rRNA gene sequence similarity value. The major cellular fatty acids of strain X7 were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 1ω9c, iso-C17 : 1ω9c, C17 : 0 2-OH and iso-C17 : 0 3-OH. The major polar lipids of strain X7 were phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids and five unidentified lipids. The predominant respiratory quinone was MK-6, and the genomic DNA G+C content was 46.7 mol%. Phylogenetic, phenotypic, chemotaxonomic and genotypic results indicated that strain X7 represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium flavum sp. nov. is proposed. The type strain is X7 (=KCTC 42585=MCCC 1H00115).
从中国威海海岸采集的海洋沉积物中分离出一株新型革兰氏阴性、不滑动、兼性厌氧的杆状细菌，命名为X7。菌株X7在28-30 °C、pH 7.0以及2-3 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列分析，菌株X7是盐微菌属的成员，与盐沼盐微菌（Salinimicrobium gaetbulicola）的16S rRNA基因序列相似性值为96.3 %，关系最为密切。菌株X7的主要细胞脂肪酸为anteiso-C15 : 0、iso-C15 : 0、anteiso-C17 : 1ω9c、iso-C17 : 1ω9c、C17 : 0 2-OH和iso-C17 : 0 3-OH。菌株X7的主要极性脂类为磷脂酰乙醇胺、一种未鉴定的磷脂、两种未鉴定的氨基脂类和五种未鉴定的脂类。主要呼吸醌为MK-6，基因组DNA的G+C含量为46.7 mol%。系统发育、表型、化学分类和基因型结果表明，菌株X7代表盐微菌属的一个新物种，为此提出盐黄盐微菌（Salinimicrobium flavum sp. nov.）这一名称。模式菌株为X7（=KCTC 42585=MCCC 1H00115）。
