危地马拉半城市日托中心儿童贾第虫病的患病率及3种贾第虫检测试验的比较
Prevalence of giardiasis in children attending semi-urban daycare centres in Guatemala and comparison of 3 giardia detection tests.
作者信息
Duffy Terri-Lynn, Montenegro-Bethancourt Gabriela, Solomons Noel W, Belosevic Miodrag, Clandinin M Thomas
机构信息
Department of Agriculture, Food and Nutritional Science, University of Alberta, Canada.
出版信息
J Health Popul Nutr. 2013 Jun;31(2):290-3. doi: 10.3329/jhpn.v31i2.16394.
Abstract
Giardia intestinalis is an intestinal parasite widely prevalent in children attending daycare centres worldwide and has been associated with undernutrition. Stool samples from 48 Guatemalan children (aged 1.5-7 years) attending participating daycare centres were analyzed over five weeks for presence of Giardia intestinalis using light microscopy, ELISA, and rapid dipstick test. Giardia prevalence rates were 43.7% at Week 0 and 44.7% at Week 4, based on ELISA. Intensity, but not prevalence, of infection showed a trend toward decreased weight-for-age (1-tailed p = 0.08). We believe that ELISA analysis of stool samples may be further adapted for measuring the intensity of infection in humans.
摘要
肠贾第虫是一种肠道寄生虫，在世界各地日托中心的儿童中广泛流行，并且与营养不良有关。在五周的时间里，对来自危地马拉参与调查的日托中心的48名儿童（年龄在1.5至7岁之间）的粪便样本进行分析，采用光学显微镜、酶联免疫吸附测定（ELISA）和快速试纸检测法检测是否存在肠贾第虫。基于ELISA检测，第0周的贾第虫感染率为43.7%，第4周为44.7%。感染强度而非感染率显示出年龄别体重下降的趋势（单尾p = 0.08）。我们认为，粪便样本的ELISA分析可能会进一步改进，以用于测量人类的感染强度。
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