Figueiredo L T, Shope R E
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510.
J Virol Methods. 1987 Sep;17(3-4):191-8. doi: 10.1016/0166-0934(87)90129-7.
A simple enzyme immunoassay (EIA) for dengue virus antibody detection used infected tissue culture cells as antigen. C6/36 Aedes albopictus cells infected with dengue virus, and uninfected control cells were fixed with 3.3% formalin. This technique eliminated microplate coating and laborious antigen preparation, and it facilitated rapid screening of large numbers of sera. Formalin also inactivated dengue virus infectivity. Microplates prepared by this technique could be stored at -20 or -70 degrees C for at least two months. Human sera were adsorbed with C6/36 cells prior to testing in the EIA in order to reduce nonspecific binding to C6/36 cells.
一种用于检测登革病毒抗体的简单酶免疫测定（EIA）方法以感染的组织培养细胞作为抗原。用登革病毒感染的白纹伊蚊C6/36细胞以及未感染的对照细胞用3.3%的福尔马林固定。该技术省去了微孔板包被和繁琐的抗原制备过程，便于快速筛查大量血清。福尔马林还使登革病毒失去感染性。用该技术制备的微孔板可在-20或-70℃下保存至少两个月。在EIA检测前，用人血清与C6/36细胞吸附，以减少与C6/36细胞的非特异性结合。
