Bokore Firdissa E, Cuthbert Richard D, Knox Ron E, Randhawa Harpinder S, Hiebert Colin W, DePauw Ron M, Singh Asheesh K, Singh Arti, Sharpe Andrew G, N'Diaye Amidou, Pozniak Curtis J, McCartney Curt, Ruan Yuefeng, Berraies Samia, Meyer Brad, Munro Catherine, Hay Andy, Ammar Karim, Huerta-Espino Julio, Bhavani Sridhar
Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, SK, S9H 3X2, Canada.
Lethbridge Research and Development Center, Agriculture and Agri-Food Canada, 5403 1st Avenue South, Lethbridge, AB, T1J 4B1, Canada.
Theor Appl Genet. 2017 Dec;130(12):2617-2635. doi: 10.1007/s00122-017-2980-7. Epub 2017 Sep 14.
Quantitative trait loci controlling stripe rust resistance were identified in adapted Canadian spring wheat cultivars providing opportunity for breeders to stack loci using marker-assisted breeding. Stripe rust or yellow rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is a devastating disease of common wheat (Triticum aestivum L.) in many regions of the world. The objectives of this research were to identify and map quantitative trait loci (QTL) associated with stripe rust resistance in adapted Canadian spring wheat cultivars that are effective globally, and investigate opportunities for stacking resistance. Doubled haploid (DH) populations from the crosses Vesper/Lillian, Vesper/Stettler, Carberry/Vesper, Stettler/Red Fife and Carberry/AC Cadillac were phenotyped for stripe rust severity and infection response in field nurseries in Canada (Lethbridge and Swift Current), New Zealand (Lincoln), Mexico (Toluca) and Kenya (Njoro), and genotyped with SNP markers. Six QTL for stripe rust resistance in the population of Vesper/Lillian, five in Vesper/Stettler, seven in Stettler/Red Fife, four in Carberry/Vesper and nine in Carberry/AC Cadillac were identified. Lillian contributed stripe rust resistance QTL on chromosomes 4B, 5A, 6B and 7D, AC Cadillac on 2A, 2B, 3B and 5B, Carberry on 1A, 1B, 4A, 4B, 7A and 7D, Stettler on 1A, 2A, 3D, 4A, 5B and 6A, Red Fife on 2D, 3B and 4B, and Vesper on 1B, 2B and 7A. QTL on 1A, 1B, 2A, 2B, 3B, 4A, 4B, 5B, 7A and 7D were observed in multiple parents. The populations are compelling sources of recombination of many stripe rust resistance QTL for stacking disease resistance. Gene pyramiding should be possible with little chance of linkage drag of detrimental genes as the source parents were mostly adapted cultivars widely grown in Canada.
在适应性良好的加拿大春小麦品种中鉴定出了控制条锈病抗性的数量性状位点，这为育种者利用标记辅助育种累加这些位点提供了机会。条锈病或黄锈病由条形柄锈菌小麦专化型（Puccinia striiformis Westend. f. sp. tritici Erikss.）引起，是世界许多地区普通小麦（Triticum aestivum L.）的一种毁灭性病害。本研究的目的是在适应性良好且在全球范围内有效的加拿大春小麦品种中鉴定并定位与条锈病抗性相关的数量性状位点（QTL），并研究累加抗性的机会。对Vesper/Lillian、Vesper/Stettler、Carberry/Vesper、Stettler/Red Fife和Carberry/AC Cadillac杂交组合产生的双单倍体（DH）群体在加拿大（莱斯布里奇和斯威夫特卡伦特）、新西兰（林肯）、墨西哥（托卢卡）和肯尼亚（恩乔罗）的田间苗圃中进行条锈病严重程度和感染反应表型分析，并用SNP标记进行基因分型。在Vesper/Lillian群体中鉴定出6个条锈病抗性QTL，Vesper/Stettler群体中有5个，Stettler/Red Fife群体中有7个，Carberry/Vesper群体中有4个，Carberry/AC Cadillac群体中有9个。Lillian在4B、5A、6B和7D染色体上贡献了条锈病抗性QTL，AC Cadillac在2A、2B、3B和5B染色体上，Carberry在1A、1B、4A、4B、7A和7D染色体上，Stettler在1A、2A、3D、4A、5B和6A染色体上，Red Fife在2D、3B和4B染色体上，Vesper在1B、2B和7A染色体上。在多个亲本中观察到了位于1A、1B、2A、2B、3B、4A、4B、5B、7A和7D染色体上的QTL。这些群体是许多条锈病抗性QTL进行重组以累加抗病性的有力来源。由于源亲本大多是在加拿大广泛种植的适应性品种，因此基因聚合应该是可行的，且有害基因连锁累赘的可能性很小。
