T细胞受体β链多态性与胰岛素依赖型糖尿病相关。
T cell receptor beta chain polymorphisms are associated with insulin-dependent diabetes.
作者信息
Millward B A, Welsh K I, Leslie R D, Pyke D A, Demaine A G
机构信息
Department of Molecular Immunogenetics and Tissue Typing, Guy's Hospital, London, UK.
出版信息
Clin Exp Immunol. 1987 Oct;70(1):152-7.
We investigated the T cell receptor constant beta chain (TCR C beta) genes of patients with insulin dependent diabetes mellitus (IDDM) using DNA restriction fragment length polymorphism (RFLP) analysis. Genomic DNA from patients and controls was digested with the restriction endonuclease Bg1 II and transferred to nylon filters using the Southern blot procedure. This enzyme identifies a polymorphic site between the two TCR C beta chain genes. We have found a highly significant increase in the frequency of the 10.0; 9.2 kilobase heterozygous phenotype in patients with IDDM (62.7% versus 42.0% in normal controls; P = 0.0006). In identical twin pairs, this association was most striking in those concordant for IDDM (79.2% versus 42.0% in controls; P = 0.0006).
我们采用DNA限制性片段长度多态性（RFLP）分析方法，对胰岛素依赖型糖尿病（IDDM）患者的T细胞受体恒定β链（TCR Cβ）基因进行了研究。用限制性内切酶Bg1 II消化患者和对照的基因组DNA，并通过Southern印迹法将其转移至尼龙滤膜上。该酶可识别两个TCR Cβ链基因之间的一个多态性位点。我们发现，IDDM患者中10.0；9.2千碱基杂合子表型的频率显著增加（62.7%，而正常对照为42.0%；P = 0.0006）。在同卵双胞胎中，这种关联在患IDDM的双胞胎中最为显著（79.2%，而对照为42.0%；P =
0.0006）。
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