基于高效液相色谱法和近红外光谱指纹图谱对不同形态乌药的分析

[Analysis of different forms Linderae Radix based on HPLC and NIRS fingerprints].

作者信息

Du Wei-Feng, Yue Xian-Ke, Wu Yao, Ge Wei-Hong, Lu Tu-Lin, Wang Zhi-Min

机构信息

Research Center of Traditonal Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China.

Zhejiang Chinese Medical University Chinese Midicine Yinpian Co., Ltd., Hangzhou 311401, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2016 Oct;41(19):3551-3556. doi: 10.4268/cjcmm20161909.

DOI:10.4268/cjcmm20161909

PMID:28925147

Abstract

Three different forms of Linderae Radix were evaluated by HPLC combined with NIRS fingerprint. The Linderae Radix was divided into three forms, including spindle root, straight root and old root. The HPLC fingerprints were developed, and then cluster analysis was performed using the SPSS software. The near-infrared spectra of Linderae Radix was collected, and then established the discriminant analysis model. The similarity values of the spindle root and straight root all were above 0.990, while the similarity value of the old root was less than 0.850. Two forms of Linderae Radix were obviously divided into three parts by the NIRS model and Cluster analysis. The results of HPLC and FT-NIR analysis showed the quality of Linderae Radix old root was different from the spindle root and straight root. The combined use of the two methods could identify different forms of Linderae Radix quickly and accurately.

摘要

采用高效液相色谱法（HPLC）结合近红外光谱（NIRS）指纹图谱对三种不同形态的乌药进行了评价。乌药分为三种形态，包括纺锤根、直根和老根。建立了HPLC指纹图谱，然后使用SPSS软件进行聚类分析。采集了乌药的近红外光谱，然后建立判别分析模型。纺锤根和直根的相似度值均在0.990以上，而老根的相似度值小于0.850。通过NIRS模型和聚类分析，两种形态的乌药明显分为三部分。HPLC和傅里叶变换近红外光谱（FT-NIR）分析结果表明，乌药老根的质量与纺锤根和直根不同。两种方法联合使用能够快速、准确地鉴别不同形态的乌药。