Ouedraogo Vincent, Kiendrebeogo Martin
Laboratoire de Biochimie & Chimie Appliquées, Université Ouaga 1 Pr. Joseph KI-ZERBO, Ouagadougou, 03 BP 7021, Burkina Faso.
Medicines (Basel). 2016 Oct 6;3(4):26. doi: 10.3390/medicines3040026.
Due to its extensive arsenal of virulence factors and inherent resistance to antibiotics, is a threat particularly in immunocompromised patients. Considering the central role of quorum sensing in the production of virulence factors, inhibition of bacterial communication mechanism constitute an opportunity to attenuate pathogenicity of bacteria resistant to available antibiotics. Our study aimed to assess the anti-quorum sensing activity of , traditionally used in Burkina Faso, for the treatment of infected burn wounds. Investigations were carried out on methanol extract from stem bark. The reporter strains CV026 and PAO1 derivatives were used to evidence any interference with the bacterial quorum sensing and expression of related genes. PAO1 was used to measure the impact on pyocyanin production. At a sub-inhibitory concentration (100 µg/mL), methanol extract quenched the quorum sensing mechanism of PAO1 by down-streaming the gene, with a subsequent reduction of pyocyanin production. Moreover, the antioxidant polyphenols evidenced are able to reduce the oxidative stress induced by pyocyanin. The antioxidant and anti-quorum sensing activities of stem bark could justify its traditional use in the treatment of infected burn wounds.
由于其丰富的毒力因子库以及对抗生素的固有抗性，尤其是在免疫功能低下的患者中构成威胁。考虑到群体感应在毒力因子产生中的核心作用，抑制细菌通讯机制为减弱对现有抗生素耐药的细菌的致病性提供了契机。我们的研究旨在评估布基纳法索传统上用于治疗感染烧伤创面的[植物名称未给出]的群体感应抑制活性。对[植物名称未给出]茎皮的甲醇提取物进行了研究。使用报告菌株CV026和铜绿假单胞菌PAO1衍生物来证明对细菌群体感应及相关基因表达的任何干扰。使用铜绿假单胞菌PAO1来测量对绿脓菌素产生的影响。在亚抑制浓度（100μg/mL）下，[植物名称未给出]甲醇提取物通过下调[基因名称未给出]基因来淬灭铜绿假单胞菌PAO1的群体感应机制，随后绿脓菌素产生减少。此外，已证明的抗氧化多酚能够减轻绿脓菌素诱导的氧化应激。[植物名称未给出]茎皮的抗氧化和群体感应抑制活性可以解释其在治疗感染烧伤创面中的传统用途。
