Mohamed Dalia A, Abdelfattah Maha I, Aboulezz Eman H A
Endodontic Department, Faculty of Dentistry Suez Canal University, Egypt.
Oro-dental Genetics Department, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt.
Open Access Maced J Med Sci. 2017 Jul 27;5(5):657-663. doi: 10.3889/oamjms.2017.089. eCollection 2017 Aug 15.
Biomaterial cytotoxicity on dental stem cells plays a critical role in managing the regeneration of dental tissue.
The aim of the present study was to evaluate the effect of Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), and Calcium-enriched mixture (CEM) on the proliferation, and viability of human dental pulp stem cells (hDPSCs) isolated from third molar teeth.
Cultured DPSCs were characterized and the tested biomaterials were shaped into cylinders then inserted directly on the DPSCs. Proliferation and viability percentage of DPSCs were evaluated at 1, 3, 5, 7, 9, 11, and 14 days of culture.
The biomaterials supplemented DPSCs showed a significant initial decrease in cell count and viability percentage at day one. Then, a rise in cell counts and viabilities was noticed after that. There was a decrease in cell counts, and viabilities in the NHA supplemented cells in comparison to other tested biomaterials.
All tested biomaterials maintain the proliferation of DPSCs for different durations. NHA showed less proliferative and more cytotoxic effect than other tested materials.
生物材料对牙干细胞的细胞毒性在牙组织再生管理中起着关键作用。
本研究旨在评估纳米羟基磷灰石（NHA）、三氧化矿物凝聚体（MTA）和富钙混合物（CEM）对从第三磨牙分离的人牙髓干细胞（hDPSCs）增殖和活力的影响。
对培养的DPSCs进行表征，将测试的生物材料制成圆柱体，然后直接插入DPSCs上。在培养的第1、3、5、7、9、11和14天评估DPSCs的增殖和活力百分比。
补充生物材料的DPSCs在第1天细胞计数和活力百分比显著初始下降。然后，之后细胞计数和活力有所上升。与其他测试生物材料相比，补充NHA的细胞中细胞计数和活力有所下降。
所有测试生物材料在不同时间段维持DPSCs的增殖。与其他测试材料相比，NHA显示出较低的增殖性和更高的细胞毒性作用。
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