Sadrabad Zahra Khalili, Habibivand Ehsan, Samiei Mohammad, Shadkar Mohammad Mostafa, Farzi Salar
Department of Pediatric Dentistry, Shahid Beheshti Dentistry Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Stem cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Conserv Dent Endod. 2025 Jan;28(1):57-62. doi: 10.4103/JCDE.JCDE_666_24. Epub 2025 Jan 13.
Regenerative therapies are emerging as promising alternatives to traditional dental treatments, aiming to preserve pulp vitality. Identifying an ideal biomaterial is crucial for the success of these therapies.
This study is the first to evaluate and compare the toxicity of nanohydroxyapatite (NHA), NHA-lactoferrin (NHA-LF), calcium-enriched mixture (CEM), and mineral trioxide aggregate (MTA) on dental pulp stem cells.
Hydroxyapatite nanocrystals were functionalized with LF in a HEPES buffer to create the NHA-LF combination. MTA, CEM, and NHA were prepared under sterile conditions. The cytotoxicity was evaluated using the MTT assay. Two-way ANOVA was used for data analysis. Games-Howell and Sidak tests were utilized for further analysis post-ANOVA.
The highest cytotoxicity was observed with NHA-LF at a 1000% concentration after 48 h, averaging 45.68%. In contrast, the lowest cytotoxicity was noted with MTA at a 10% concentration within 24 h, averaging 229.53%. There was a significant statistical relationship between substance type and cellular viability (P < 0.05), whereas time showed no significant impact (P > 0.05).
The findings indicate a significant correlation between the type of biomaterial used and its effect on cellular proliferation and viability, with time showing no significant influence. All the tested materials can be categorized as noncytotoxic materials, except NHA-LF in the highest concentration (1000%). The study suggests prioritizing low-dose NHA-LF for use.
再生疗法正成为传统牙科治疗的有前景的替代方法，旨在保留牙髓活力。确定理想的生物材料对这些疗法的成功至关重要。
本研究首次评估并比较纳米羟基磷灰石（NHA）、NHA-乳铁蛋白（NHA-LF）、富钙混合物（CEM）和三氧化矿物凝聚体（MTA）对牙髓干细胞的毒性。
在HEPES缓冲液中用乳铁蛋白对羟基磷灰石纳米晶体进行功能化处理，以制备NHA-LF组合。MTA、CEM和NHA在无菌条件下制备。使用MTT法评估细胞毒性。采用双向方差分析进行数据分析。方差分析后，使用Games-Howell和Sidak检验进行进一步分析。
48小时后，1000%浓度的NHA-LF观察到最高细胞毒性，平均为45.68%。相比之下，24小时内10%浓度的MTA观察到最低细胞毒性，平均为229.53%。物质类型与细胞活力之间存在显著的统计学关系（P < 0.05），而时间显示无显著影响（P > 0.05）。
研究结果表明，所用生物材料的类型与其对细胞增殖和活力的影响之间存在显著相关性，时间无显著影响。除最高浓度（1000%）的NHA-LF外，所有测试材料均可归类为无细胞毒性材料。该研究建议优先使用低剂量的NHA-LF。
