Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg (FAU), Erlangen, Germany.
Institute of Polymer Materials, Friedrich-Alexander-University of Erlangen-Nürnberg (FAU), Erlangen, Germany.
Biomed Res Int. 2017;2017:9616939. doi: 10.1155/2017/9616939. Epub 2017 Aug 28.
The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron®, or Teflon® still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the focus of research to imitate the extracellular matrix (ECM) of vessels to produce a small caliber, off-the-shelf tissue engineered vascular graft (TEVG) as a substitute for poorly performing PU, Dacron, or Teflon prostheses. Blended poly--caprolactone (PCL)/collagen grafts have shown promising results regarding biomechanical and cell supporting features. In order to find a suitable PCL/collagen blend, we fabricated plane electrospun PCL scaffolds using various collagen type I concentrations ranging from 5% to 75%. We analyzed biocompatibility and morphological aspects . Our results show beneficial features of collagen I integration regarding cell viability and functionality, but also adverse effects like the loss of a confluent monolayer at high concentrations of collagen. Furthermore, electrospun PCL scaffolds containing 25% collagen I seem to be ideal for engineering vascular grafts.
血管移植物的工程是再生医学中一个不断发展的领域。尽管已经进行了多次尝试，但目前由聚氨酯（PU）、Dacron®或 Teflon®制成的血管移植物仍然显示出令人不满意的结果。生物聚合物和天然蛋白质的静电纺丝一直是研究的重点，旨在模仿血管的细胞外基质（ECM），以生产小口径、现成的组织工程血管移植物（TEVG），作为性能不佳的 PU、Dacron 或 Teflon 假体的替代品。混合聚--己内酯（PCL）/胶原移植物在生物力学和细胞支持特性方面显示出有希望的结果。为了找到合适的 PCL/胶原混合物，我们使用从 5%到 75%的各种胶原 I 浓度制造平面静电纺丝 PCL 支架。我们分析了生物相容性和形态方面。我们的结果表明胶原 I 整合具有细胞活力和功能的有益特征，但在胶原浓度高时也会产生不利影响，如失去细胞单层的融合。此外，含有 25%胶原 I 的静电纺丝 PCL 支架似乎是工程血管移植物的理想选择。
