[长春花的密码子使用偏好性]
[Codon usage bias of Catharanthus roseus].
作者信息
Li Ying, Kuang Xue-Jun, Zhu Xiao-Xuan, Zhu Ying-Jie, Sun Chao
机构信息
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4165-4168. doi: 10.4268/cjcmm20162213.
This study aimed to provide guidance for the heterogenous gene expression, gene prediction and species evolution by analyzing codon usage bias of Catharanthus roseus.The codon composition and usage bias of 30 437 high-confidence coding sequences from C.roseus were analyzed and the proportion of rare codons of Escherichia coli and Saccharomyces cerevisiae in 25 genes involved in the biosynthesis of terpenoid indole alkaloids (TIAs) in C.roseus were calculated.The results showed that the average GC content of the genes was 42.47%; the average GC content of the third bases in codon was 35.89%.The relative synonymous codon usage (RSCU) of 28 codons were greater than 1 and 26 of them ended with A or T.The above 25 genes involved in TIA biosynthesis contained much more rare condons of E.coli than that of S.cerevisiae.It was concluded that C.roseus mainly prefered the codons ending with A or T and the rule of codon usage was more different to E.coli than S.cerevisiae.Thus, S.cerevisiae may be more suitable host for heterologous expression of these genes.
本研究旨在通过分析长春花密码子使用偏好，为其基因表达异质性、基因预测及物种进化提供指导。分析了长春花30437条高可信度编码序列的密码子组成及使用偏好，并计算了长春花中25个参与萜类吲哚生物碱（TIAs）生物合成的基因中大肠杆菌和酿酒酵母稀有密码子的比例。结果表明，这些基因的平均GC含量为42.47%；密码子第三位碱基的平均GC含量为35.89%。28个密码子的相对同义密码子使用度（RSCU）大于1，其中26个以A或T结尾。上述25个参与TIAs生物合成的基因中，大肠杆菌稀有密码子的含量远多于酿酒酵母。得出结论：长春花主要偏好以A或T结尾的密码子，其密码子使用规则与大肠杆菌的差异大于与酿酒酵母的差异。因此，酿酒酵母可能是这些基因异源表达更合适的宿主。
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