Environmental Biology Laboratory, P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, 580003, India.
Environ Sci Pollut Res Int. 2017 Nov;24(33):25861-25869. doi: 10.1007/s11356-017-9899-z. Epub 2017 Sep 21.
Silver nanoparticles synthesized using the leaf extract of Albizia saman (Jacq.) Merr. were tested for induction of cytogenetic abnormality in root tip cells of Drimia indica (Roxb.) Jessop (family Asperagaceae). The leaves are known to be rich in various phytochemicals like flavonoids, glycosides, saponins, steroids, tannins, and terpenoids, which may be responsible for bioreduction, biocapping, and stabilization of nanoparticles. The various instruments used for characterization include UV-VIS spectrophotometer, fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), X-Ray diffractometer (XRD), and high resolution transmission electron microscope (HR-TEM). The present study aims to evaluate the cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes by using root tip cells of D. indica. The root tips of D. indica was treated with suspensions of silver nanoparticles mixed in distilled water at different concentrations viz., 25, 50, 75, and 100% (w/v) for 6, 12, 18, and 24 h and then fixed in 1:3 ethanol: acetic acid following pre-treatment with 0.05% colchicine for cytological analysis. Silver nanoparticles induced a dose dependent decrease of mitotic index in root meristems. Furthermore, the treated meristem cells showed various types of chromosomal and mitotic aberrations such as anaphase bridge, sticky metaphase, lagging, or forward chromosome indicating genotoxic damage.
采用银合欢（Albizia saman（Jacq.）Merr.）叶提取物合成的银纳米粒子，用于测试其对印度杜兰（Drimia indica（Roxb.）Jessop）根尖细胞（天门冬科）遗传细胞学异常的诱导作用。众所周知，该植物的叶子富含各种植物化学物质，如类黄酮、糖苷、皂苷、类固醇、单宁和萜类化合物，这些物质可能负责纳米粒子的生物还原、生物帽和稳定。用于表征的各种仪器包括紫外-可见分光光度计、傅里叶变换红外光谱（FTIR）、原子力显微镜（AFM）、X 射线衍射仪（XRD）和高分辨率透射电子显微镜（HR-TEM）。本研究旨在通过印度杜兰根尖细胞评估生物合成银纳米粒子对有丝分裂染色体的细胞毒性作用。将印度杜兰的根尖用悬浮在蒸馏水中的银纳米粒子混合物处理，浓度分别为 25、50、75 和 100%（w/v），处理时间分别为 6、12、18 和 24 小时，然后用 0.05%秋水仙素进行预处理，用 1:3 的乙醇：乙酸固定，用于细胞学分析。银纳米粒子诱导有丝分裂指数在根尖分生组织中呈剂量依赖性下降。此外，处理的分生组织细胞显示出各种类型的染色体和有丝分裂异常，如后期桥、粘性中期、滞后或向前染色体，表明遗传毒性损伤。
