Karimoei Mostafa, Pasalar Parvin, Mehrabzadeh Mohsen, Daneshpour Maryam, Shojaee Maryam, Forouzanfar Katayoun, Razi Farideh
Department of Medical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran.
Endocrine and Metabolic Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Saudi J Kidney Dis Transpl. 2017 Sep-Oct;28(5):997-1002. doi: 10.4103/1319-2442.215137.
Approximately one-third of diabetic patients develop evidence of nephropathy. Pathogenesis of diabetic nephropathy (DN) remains unclear; however, some genetic and metabolic risk factors have been determined for the development and progression of DN. In the recent genetic studies, polymorphism of apolipoprotein E (ApoE) gene has been reported as a risk factor for the development of DN; however, the results are inconsistent. The aim of the present study was to evaluate the association between ApoE polymorphism and nephropathy in Iranian patient with type 2 diabetes. A total of 197 patients with type 2 diabetes in two groups with and without nephropathy (n = 99 and n = 98, respectively) participated in this case-control study. ApoE genotype was determined by restriction fragment length polymorphism analysis. Biochemical factors of all patients were measured. The frequency of Apo ε4 allele was significantly (P <0.05) lower in DN patients (10.6%) than in diabetic patients without nephropathy (20.4%). No significant difference was observed between the groups regarding Apo ε2 and Apo ε3 allele frequencies. Serum level of total and low-density lipoprotein cholesterol in Apo ε2 carriers was lower than Apo ε3 and Apo ε4 carriers, but this difference was not statistically significant. Frequency of Apo ε4 allele is higher in diabetic patients without nephropathy than DN participants. Given to the result, it seems that Apo ε4 has a protective effect in diabetic patients against nephropathy.
约三分之一的糖尿病患者会出现肾病迹象。糖尿病肾病（DN）的发病机制尚不清楚；然而，一些遗传和代谢风险因素已被确定与DN的发生和发展有关。在最近的基因研究中，载脂蛋白E（ApoE）基因多态性被报道为DN发生的一个风险因素；然而，结果并不一致。本研究的目的是评估伊朗2型糖尿病患者中ApoE多态性与肾病之间的关联。共有197例2型糖尿病患者参与了这项病例对照研究，分为有肾病组和无肾病组（分别为n = 99和n = 98）。通过限制性片段长度多态性分析确定ApoE基因型。测量了所有患者的生化指标。DN患者中Apo ε4等位基因的频率（10.6%）显著低于无肾病的糖尿病患者（20.4%）（P <0.05）。两组之间在Apo ε2和Apo ε3等位基因频率方面未观察到显著差异。Apo ε2携带者的总胆固醇和低密度脂蛋白胆固醇血清水平低于Apo ε3和Apo ε4携带者，但这种差异无统计学意义。无肾病的糖尿病患者中Apo ε4等位基因的频率高于DN患者。基于该结果，似乎Apo ε4对糖尿病患者预防肾病具有保护作用。
