Asgarbeik Saeedeh, Mohammad Amoli Mahsa, Enayati Samaneh, Bandarian Fatemeh, Nasli-Esfahani Ensieh, Forouzanfar Katayoon, Razi Farideh, Angaji Seyed Abdolhamid
Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Int J Mol Cell Med. 2019 Winter;8(Suppl1):49-55. doi: 10.22088/IJMCM.BUMS.8.2.49. Epub 2019 Jun 15.
Nephropathy is a common diabetes complication. gene which participates in various cellular pathways has been proposed as a candidate gene in diabetic nephropathy. This study aimed to investigate the role of +808T/G polymorphism (rs377349) in gene in diabetic nephropathy. In this case-control study, patients including diabetes with nephropathy (DN=104), type 2 diabetes without nephropathy (DM=100), and healthy controls (HC=106) were included. DNA was extracted from blood, and genotyping of the +808T/G polymorphism was carried out using PCR-RFLP technique. The differences for genotype and allele frequencies for +808T/G polymorphism in gene between DN vs. HC and DN+DM vs. HC were significant (P<0.05) while no significant difference between DN and DM was observed. The allele frequencies were significantly different in DN vs. HC and DN+DM vs. HC in males but not in females. G allele of +808T/G polymorphism in gene has no significant role in development and progression of diabetic nephropathy in diabetes patients while it is a risk allele for developing diabetes in Iranian population.
肾病是一种常见的糖尿病并发症。参与多种细胞途径的基因已被提出作为糖尿病肾病的候选基因。本研究旨在探讨该基因中+808T/G多态性（rs377349）在糖尿病肾病中的作用。在这项病例对照研究中，纳入了患有肾病的糖尿病患者（DN = 104）、无肾病的2型糖尿病患者（DM = 100）和健康对照者（HC = 106）。从血液中提取DNA，并使用PCR-RFLP技术对+808T/G多态性进行基因分型。DN与HC以及DN + DM与HC之间该基因中+808T/G多态性的基因型和等位基因频率差异具有统计学意义（P < 0.05），而DN与DM之间未观察到显著差异。男性中DN与HC以及DN + DM与HC的等位基因频率存在显著差异，而女性中无显著差异。该基因中+808T/G多态性的G等位基因在糖尿病患者糖尿病肾病的发生和发展中无显著作用，但在伊朗人群中它是患糖尿病的一个风险等位基因。
