罗西奥病毒的全基因组序列显示出与原型罗西奥病毒SPH 34675序列存在显著差异。

Full genome sequence of Rocio virus reveal substantial variations from the prototype Rocio virus SPH 34675 sequence.

作者信息

Setoh Yin Xiang, Amarilla Alberto A, Peng Nias Y, Slonchak Andrii, Periasamy Parthiban, Figueiredo Luiz T M, Aquino Victor H, Khromykh Alexander A

机构信息

Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, 4072, Australia.

Virology Research Center, School of Medicine of Ribeirao Preto, University of de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.

出版信息

Arch Virol. 2018 Jan;163(1):255-258. doi: 10.1007/s00705-017-3561-4. Epub 2017 Sep 22.

DOI:10.1007/s00705-017-3561-4

PMID:28939977

Abstract

Rocio virus (ROCV) is an arbovirus belonging to the genus Flavivirus, family Flaviviridae. We present an updated sequence of ROCV strain SPH 34675 (GenBank: AY632542.4), the only available full genome sequence prior to this study. Using next-generation sequencing of the entire genome, we reveal substantial sequence variation from the prototype sequence, with 30 nucleotide differences amounting to 14 amino acid changes, as well as significant changes to predicted 3'UTR RNA structures. Our results present an updated and corrected sequence of a potential emerging human-virulent flavivirus uniquely indigenous to Brazil (GenBank: MF461639).

摘要

罗西奥病毒（ROCV）是一种属于黄病毒属、黄病毒科的虫媒病毒。我们展示了ROCV毒株SPH 34675的更新序列（GenBank：AY632542.4），这是本研究之前唯一可用的全基因组序列。通过对整个基因组进行二代测序，我们发现该序列与原型序列存在大量变异，有30个核苷酸差异，相当于14个氨基酸变化，并且预测的3'UTR RNA结构也有显著变化。我们的结果展示了一种潜在的新出现的、对人类具有致病性的黄病毒的更新且校正后的序列，该病毒是巴西独有的本土病毒（GenBank：MF461639）。