Garduno F, Thorne L, Walfield A M, Pollock T J
Syntro Corporation, San Diego, California 92121.
Appl Environ Microbiol. 1988 Jan;54(1):277-9. doi: 10.1128/aem.54.1.277-279.1988.
A mosquitocidal toxin gene, cloned from Bacillus thuringiensis subsp. israelensis, was introduced into mutant crystal-negative B. thuringiensis subsp. israelensis cells. Partial toxicity to mosquitos was restored. The 58-kilodalton cloned gene product is a minor protein component of B. thuringiensis subsp. israelensis crystals and is structurally related to a major, 135-kilodalton crystal toxin.
从苏云金芽孢杆菌以色列亚种克隆出的一种杀蚊毒素基因,被导入到突变的无晶体苏云金芽孢杆菌以色列亚种细胞中。对蚊子的部分毒性得以恢复。这个58千道尔顿的克隆基因产物是苏云金芽孢杆菌以色列亚种晶体中的一种次要蛋白质成分,并且在结构上与一种主要的135千道尔顿晶体毒素相关。