Sae-Leaw Thanasak, Benjakul Soottawat
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Food Chem. 2018 Feb 1;240:9-15. doi: 10.1016/j.foodchem.2017.07.089. Epub 2017 Jul 19.
Lipase from liver of seabass (Lates calcarifer), with a molecular weight of 60kDa, was purified to homogeneity using ammonium sulfate precipitation and a series of chromatographies, including diethylaminoethyl sepharose (DEAE) and Sephadex G-75 size exclusion columns. The optimal pH and temperature were 8.0 and 50°C, respectively. Purified lipase had Michaelis-Menten constant (K) and catalytic constant (k) of 0.30mM and 2.16s, respectively, when p-nitrophenyl palmitate (p-NPP) was used as the substrate. When seabass skin was treated with crude lipase from seabass liver at various levels (0.15 and 0.30units/g dry skin) for 1-3h at 30°C, the skin treated with lipase at 0.30 units/g dry skin for 3h had the highest lipid removal (84.57%) with lower lipid distribution in skin. Efficacy in defatting was higher than when isopropanol was used. Thus, lipase from liver of seabass could be used to remove fat in fish skin.
来自尖吻鲈（Lates calcarifer）肝脏的脂肪酶，分子量为60kDa，通过硫酸铵沉淀和一系列色谱法（包括二乙氨基乙基琼脂糖（DEAE）和葡聚糖G-75尺寸排阻柱）纯化至同质。最佳pH和温度分别为8.0和50°C。当以对硝基苯基棕榈酸酯（p-NPP）为底物时，纯化的脂肪酶的米氏常数（K）和催化常数（k）分别为0.30mM和2.16s。当在30°C下用不同水平（0.15和0.30单位/克干皮）的尖吻鲈肝脏粗脂肪酶处理尖吻鲈皮肤1-3小时时，用0.30单位/克干皮的脂肪酶处理3小时的皮肤具有最高的脂质去除率（84.57%），且皮肤中的脂质分布较低。脱脂效果高于使用异丙醇时。因此，尖吻鲈肝脏中的脂肪酶可用于去除鱼皮中的脂肪。
