State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Airways Disease Section, National Heart & Lung Institute, Imperial College London, & Biomedical Research Unit, Royal Brompton Hospital, London, SW3, UK.
Respir Med. 2017 Oct;131:192-198. doi: 10.1016/j.rmed.2017.08.016. Epub 2017 Aug 25.
Altered composition of airway microbiota has been reported in subjects suffering from asthma but its relation to eosinophilic phenotype is unclear.
To examine the relationship between sputum microbiota, asthma severity and inflammatory type in asthmatic subjects from Guangzhou, China.
Induced sputum samples were obtained from 49 non-smoking asthma patients, 25 severe and 24 non-severe, and 15 healthy subjects. Total DNA was amplified using primers specific for the V3-V5 hypervariable region of bacterial 16s rRNA and sequenced using the 454 GS FLX sequencer. Sequences were assigned to bacterial taxa by comparing them with 16s rRNA sequences in the Ribosomal Database Project.
Sputum eosinophil counts were higher and FEV (% predicted) was lower in severe compared to non-severe asthmatics. There were no significant differences in operational taxonomic unit (OTU) numbers at the phylum level and in diversity scores between non-severe asthmatics and severe asthmatics, and healthy subjects. At the family level, Porphyromonadaceae was most abundant in healthy subjects whereas Pseudomonadaceae and Enterobacteriaceae were higher in severe asthmatics compared to non-severe asthmatics (p < 0.05). Actinomycetaceae was particularly abundant in eosinophilic asthma patients compared to non-eosinophilic asthma (p = 0.011). Bacteroidaceae was positively correlated with FEV in all subjects (r = 0.335, p < 0.01), whereas body mass index was negatively associated with the number of species observed (r = -0.3, p < 0.05). Principal component analysis confirmed the positive association of Actinomycetaceae and Enterobacteriaceae abundance with eosinophilic asthma.
Patients with asthma have an altered airway microbiota, with specific bacteria associated with severe asthma and the eosinophilic inflammatory phenotype.
患有哮喘的患者气道微生物群落的组成发生了改变，但其与嗜酸性粒细胞表型的关系尚不清楚。
在中国广州的哮喘患者中，研究痰微生物群落与哮喘严重程度和炎症类型之间的关系。
共纳入 49 例非吸烟哮喘患者，其中 25 例为重度哮喘患者，24 例为非重度哮喘患者，15 例为健康对照者。使用针对细菌 16s rRNA V3-V5 高变区的引物扩增诱导痰标本中的总 DNA，然后使用 454 GS FLX 测序仪进行测序。将序列与核糖体数据库项目中的 16s rRNA 序列进行比较，从而将序列分配到细菌分类群中。
与非重度哮喘患者相比，重度哮喘患者的痰嗜酸性粒细胞计数较高，FEV（%预计值）较低。非重度哮喘患者、重度哮喘患者和健康对照者的门水平操作分类单元（OTU）数量和多样性评分均无显著差异。在科水平，健康受试者中最丰富的是卟啉单胞菌科，而与非重度哮喘患者相比，重度哮喘患者中假单胞菌科和肠杆菌科更为丰富（p<0.05）。与非嗜酸性粒细胞性哮喘患者相比，嗜酸性粒细胞性哮喘患者中放线菌科特别丰富（p=0.011）。在所有受试者中，拟杆菌科与 FEV 呈正相关（r=0.335，p<0.01），而体质指数与观察到的物种数量呈负相关（r=-0.3，p<0.05）。主成分分析证实了放线菌科和肠杆菌科丰度与嗜酸性粒细胞性哮喘的正相关关系。
哮喘患者的气道微生物群落发生了改变，某些细菌与重度哮喘和嗜酸性粒细胞炎症表型相关。
