Department of Rehabilitation Medicine, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, People's Republic of China.
Core Facility of West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.
Metab Brain Dis. 2018 Feb;33(1):15-25. doi: 10.1007/s11011-017-0113-2. Epub 2017 Sep 25.
Whole body vibration (WBV) has been applied in stroke patients with uncertain effects on motor and sensory dysfunction, and its effects on neurogenesis have not been studied yet. Here, we intended to explore the effects of daily WBV on neurological behavior, brain structure, and neurogenesis after cerebral ischemia in rats for 4 weeks. Results showed that improvements in weight or comprehensive neurological deficits were not significantly different under WBV or control treatment, and the degrees of brain damage and the numbers of necrotic neurons in the ischemic cortex were similar in two groups. However, WBV markedly improved animals' coordination from 14d to 28d (P < 0.05) and muscle strength of the upper limbs at 21d and 28d (P < 0.05 & P < 0.001) compared with the control group. WBV promoted the increase in the number of bromodeoxyuridine-positive (BrdU) cells at 3d (P < 0.05) and 14d (P < 0.001) and the number of BrdU/nestin cells at 14d (P < 0.01) after ischemia when compared to the control group. The numbers of BrdU/NeuN cells at 21d and 28d (P < 0.001) were enhanced by WBV treatment. In addition, WBV significantly promoted the proliferation of astrocytes and their neural processes thickening after 14d. The expression levels of neural markers, such as doublecortin, microtubule-associated protein 2, and glial fibrillary acidic protein, were upregulated in the ipsilateral cortex at different time points. Low-frequency WBV showed inconspicuous improvements in behavioral performance and brain damage after cerebral ischemia, but showed the potential in improving coordination and muscle strength and promoted neurogenesis after long-term exposure.
全身振动（WBV）已应用于中风患者，但对运动和感觉功能障碍的影响尚不确定，其对神经发生的影响尚未研究。在这里，我们旨在探讨每天进行 WBV 对大脑缺血后 4 周大鼠的神经行为、大脑结构和神经发生的影响。结果表明，WBV 或对照组治疗对体重或综合神经缺损的改善没有显著差异，两组缺血皮质的脑损伤程度和坏死神经元数量相似。然而，与对照组相比，WBV 显著改善了动物从第 14 天到第 28 天的协调能力（P<0.05）以及第 21 天和第 28 天的上肢肌肉力量（P<0.05 和 P<0.001）。与对照组相比，WBV 促进了缺血后第 3 天（P<0.05）和第 14 天（P<0.001）BrdU 阳性细胞（BrdU）数量的增加以及第 14 天 BrdU/nestin 细胞数量的增加（P<0.01）。与对照组相比，21 天和 28 天 BrdU/NeuN 细胞数量增加（P<0.001）。此外，WBV 还显著促进了缺血后第 14 天星形胶质细胞的增殖及其神经突的增厚。在不同时间点，同侧皮质中神经标志物如双皮质蛋白、微管相关蛋白 2 和神经胶质纤维酸性蛋白的表达水平上调。低频 WBV 对脑缺血后行为表现和脑损伤的改善作用不明显，但长期暴露后具有改善协调和肌肉力量以及促进神经发生的潜力。
