基于消除反应的苯并咪唑探针用于半胱氨酸检测及其在血清样品分析中的应用。
Elimination Reaction-Based Benzimidazole Probe for Cysteine Detection and Its Application in Serum Sample Analysis.
机构信息
Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Korea.
School of Chemical Sciences, KBC-North Maharashtra University, Jalgaon 425001, India.
出版信息
Biosensors (Basel). 2022 Apr 8;12(4):224. doi: 10.3390/bios12040224.
Benzimidazole-based compound 2-(-tolyl)-1-benzo[d]imidazole () and its derivative probe have been synthesized for the highly selective detection and quantification of Cys in human serum. The photophysical properties of and compound were evaluated by UV-vis absorption and fluorescence spectroscopy. showed high selectivity and sensitivity for Cys among tested analytes, including amino acids, anions, and cations. selectively reacts with Cys and results in compound with fluorescence turn-on effect. did not show any interference from the components in the serum matrix for Cys detection in the human serum sample. detects Cys in serum samples with 2.3-5.4-fold better LOD than reported methods. The detection limit of 86 nM and 43 nM in HEPES buffer using UV-visible and fluorescence spectroscopy, respectively, makes an excellent chemosensor for Cys detection.
苯并咪唑类化合物 2-(-甲苯基)-1-苯并[d]咪唑 () 和其衍生探针 已被合成用于高度选择性检测和定量人血清中的半胱氨酸。通过紫外-可见吸收和荧光光谱法评估了 和化合物 的光物理性质。 在测试的分析物中，包括氨基酸、阴离子和阳离子， 对半胱氨酸具有高选择性和灵敏度。 选择性地与半胱氨酸反应，导致化合物 具有荧光开启效应。 在人血清样本中对半胱氨酸的检测中， 没有显示出来自血清基质成分的任何干扰。 比报道的方法对血清样品中的半胱氨酸检测具有 2.3-5.4 倍更好的检出限。使用紫外-可见和荧光光谱法在 HEPES 缓冲液中的检测限分别为 86 nM 和 43 nM，使 成为半胱氨酸检测的优秀化学传感器。
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