Reddi Nagesh Madhuranthakam, Gatasheh Mansour K, Hoda Nasrul, Vijayakumar Natesan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Department of Biochemistry, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2022 Aug;29(8):103370. doi: 10.1016/j.sjbs.2022.103370. Epub 2022 Jul 1.
Genotoxicity analysis is one of the most important non-clinical environmental safety investigations required for pharmaceutical and agrochemical product registration. Any medicinal product must undergo a risk evaluation to determine its mutagenicity and carcinogenicity.
The Ames test is a commonly used test for determining a test chemical's mutagenic activity. Histidine-dependent strains with a defective gene that causes the bacteria to synthesis the necessary amino acid histidine for life were tested for mutagenic potential. In order to reveal pro-mutagens and mutagens, the mutagenic potential of both plate integration and pre-incubation techniques was examined in the presence and absence of metabolizing system. has been widely used in ayurveda to treat various ailments. However, the information of mutagenicity of is scarce as per available literature.
The mutagenicity of a root extract was investigated utilizing the Ames assay with plate incorporation and pre-incubation protocols using the appropriate tester strains: TA98, TA100, TA1537, TA1535, and TA102 in the presence and absence of S9. The concentrations used were 0.3123, 0.625, 1.25, 2.5 and 5 mg/plate. The extract of root did not show any mutagenic effect in any of the strains at the concentrations tested in the absence or presence of metabolic activation.
The root of was hence confirmed to be non-mutagenic and at least according to the results of this genotoxicity evaluation can be regarded as being safe for human use.
遗传毒性分析是药品和农用化学品注册所需的最重要的非临床环境安全性研究之一。任何药品都必须进行风险评估，以确定其致突变性和致癌性。
艾姆斯试验是一种常用的用于确定受试化学品致突变活性的试验。对具有缺陷基因的组氨酸依赖型菌株进行测试，该缺陷基因导致细菌无法合成生命所需的必需氨基酸组氨酸，以此检测其致突变潜力。为了揭示前诱变剂和诱变剂，在有和没有代谢系统的情况下，研究了平板掺入法和预孵育技术的诱变潜力。[某种植物]在阿育吠陀医学中已被广泛用于治疗各种疾病。然而，根据现有文献，关于[该植物]致突变性的信息却很少。
利用艾姆斯试验，采用平板掺入法和预孵育方案，在有和没有S9的情况下，使用适当的测试菌株TA98、TA100、TA1537、TA1535和TA102，研究了[某种植物]根提取物的致突变性。所用浓度为0.3123、0.625、1.25、2.5和5毫克/平板。在不存在或存在代谢活化的情况下，在所测试的浓度下，[该植物]根提取物在任何测试菌株中均未显示出任何致突变作用。
因此，[该植物]的根被证实无致突变性，至少根据该遗传毒性评估的结果，可以认为对人类使用是安全的。
