• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

安普那韦对慢性感染单核细胞/巨噬细胞中药物停药后 HIV-1 成熟、产生和感染性的影响。

Effects of Amprenavir on HIV-1 Maturation, Production and Infectivity Following Drug Withdrawal in Chronically-Infected Monocytes/Macrophages.

机构信息

Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, 00133 Roma, Italy.

Clinical Virology Group, Institute of Biomedical Research of A Coruña (INIBIC)-University Hospital of A Coruña (CHUAC), Sergas, University of A Coruña (UDC), 15001 A Coruña, Spain.

出版信息

Viruses. 2017 Sep 28;9(10):277. doi: 10.3390/v9100277.

DOI:10.3390/v9100277
PMID:28956865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5691629/
Abstract

A paucity of information is available on the activity of protease inhibitors (PI) in chronically-infected monocyte-derived macrophages (MDM) and on the kinetics of viral-rebound after PI removal in vitro. To fill this gap, the activity of different concentrations of amprenavir (AMP) was evaluated in chronically-infected MDM by measuring p24-production every day up to 12 days after drug administration and up to seven days after drug removal. Clinically-relevant concentrations of AMP (4 and 20 μM) drastically decreased p24 amount released from chronically-infected MDM from Day 2 up to Day 12 after drug administration. The kinetics of viral-rebound after AMP-removal (4 and 20 μM) showed that, despite an initial increase, p24-production over time never reached the level observed for untreated-MDM, suggesting a persistent intracellular drug activity. In line with this, after AMP-removal, human immunodeficiency virus 1 (HIV-1) infectivity and intracellular the p24/p55 ratio (reflecting virion-maturation) were remarkably lower than observed for untreated MDM. Overall, AMP shows high efficacy in blocking HIV-1 replication in chronically-infected MDM, persisting even after drug-removal. This highlights the role of protease inhibitors in preventing the establishment of this important HIV-1 reservoir, thus reducing viral-dissemination in different anatomical compartments.

摘要

关于蛋白酶抑制剂（PI）在慢性感染的单核细胞衍生巨噬细胞（MDM）中的活性，以及在体外去除 PI 后病毒反弹的动力学方面，信息有限。为了填补这一空白，通过在药物给药后第 12 天和药物去除后第 7 天测量 p24 的产生，评估了不同浓度安普那韦（AMP）在慢性感染的 MDM 中的活性。临床相关浓度的 AMP（4 和 20 μM）从药物给药后第 2 天到第 12 天，大大降低了从慢性感染的 MDM 释放的 p24 量。AMP 去除（4 和 20 μM）后的病毒反弹动力学表明，尽管最初有所增加，但随着时间的推移，p24 的产生从未达到未处理的 MDM 观察到的水平，表明细胞内药物活性持续存在。与此一致的是，AMP 去除后，人类免疫缺陷病毒 1（HIV-1）感染性和细胞内 p24/p55 比值（反映病毒成熟）明显低于未处理的 MDM。总的来说，AMP 在阻断慢性感染的 MDM 中的 HIV-1 复制方面具有很高的疗效，即使在药物去除后也能持续存在。这突出了蛋白酶抑制剂在防止这种重要的 HIV-1 储存库建立方面的作用，从而减少了不同解剖部位的病毒传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/8cbb6c47b192/viruses-09-00277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/e778522e1e5b/viruses-09-00277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/785ddbdbc37c/viruses-09-00277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/2fbad274bf1c/viruses-09-00277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/1bc8ce966b8c/viruses-09-00277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/8cbb6c47b192/viruses-09-00277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/e778522e1e5b/viruses-09-00277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/785ddbdbc37c/viruses-09-00277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/2fbad274bf1c/viruses-09-00277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/1bc8ce966b8c/viruses-09-00277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f8/5691629/8cbb6c47b192/viruses-09-00277-g005.jpg

相似文献

1
Effects of Amprenavir on HIV-1 Maturation, Production and Infectivity Following Drug Withdrawal in Chronically-Infected Monocytes/Macrophages.安普那韦对慢性感染单核细胞/巨噬细胞中药物停药后 HIV-1 成熟、产生和感染性的影响。
Viruses. 2017 Sep 28;9(10):277. doi: 10.3390/v9100277.
2
Potent antiviral activity of amprenavir in primary macrophages infected by human immunodeficiency virus.安普那韦对人免疫缺陷病毒感染的原代巨噬细胞具有强大的抗病毒活性。
Antiviral Res. 2004 Feb;61(2):133-7. doi: 10.1016/j.antiviral.2003.09.002.
3
Relative potency of protease inhibitors in monocytes/macrophages acutely and chronically infected with human immunodeficiency virus.蛋白酶抑制剂对急性和慢性感染人类免疫缺陷病毒的单核细胞/巨噬细胞的相对效力。
J Infect Dis. 1998 Aug;178(2):413-22. doi: 10.1086/515642.
4
Granulocyte-macrophage colony-stimulating factor inhibits HIV-1 replication in monocyte-derived macrophages.粒细胞巨噬细胞集落刺激因子抑制HIV-1在单核细胞衍生巨噬细胞中的复制。
AIDS. 2000 Aug 18;14(12):1739-48. doi: 10.1097/00002030-200008180-00008.
5
Interference of interleukin-10 with human immunodeficiency virus type 1 replication in primary monocyte-derived macrophages.白细胞介素-10对人免疫缺陷病毒1型在原代单核细胞衍生巨噬细胞中复制的干扰作用。
J Virol. 1994 Nov;68(11):6967-75. doi: 10.1128/JVI.68.11.6967-6975.1994.
6
Phenotypic hypersusceptibility to multiple protease inhibitors and low replicative capacity in patients who are chronically infected with human immunodeficiency virus type 1.1型人类免疫缺陷病毒慢性感染患者对多种蛋白酶抑制剂的表型超敏感性及低复制能力
J Virol. 2005 May;79(10):5907-13. doi: 10.1128/JVI.79.10.5907-5913.2005.
7
Infection of human monocytes with Mycobacterium tuberculosis enhances human immunodeficiency virus type 1 replication and transmission to T cells.结核分枝杆菌感染人类单核细胞会增强1型人类免疫缺陷病毒的复制并促进其向T细胞的传播。
J Infect Dis. 1997 Jun;175(6):1531-5. doi: 10.1086/516494.
8
Cystamine inhibits HIV type 1 replication in cells of monocyte/macrophage and T cell lineages.半胱胺抑制1型人类免疫缺陷病毒在单核细胞/巨噬细胞和T细胞系细胞中的复制。
AIDS Res Hum Retroviruses. 1995 Apr;11(4):451-9. doi: 10.1089/aid.1995.11.451.
9
Inhibition of human immunodeficiency virus type-1 replication in macrophages and H9 cells by free or liposome-encapsulated L-689,502, an inhibitor of the viral protease.游离型或脂质体包裹型病毒蛋白酶抑制剂L-689,502对巨噬细胞和H9细胞中人类免疫缺陷病毒1型复制的抑制作用
Antiviral Res. 1997 Mar;34(1):1-15. doi: 10.1016/s0166-3542(96)01017-0.
10
IL-13 acts on macrophages to block the completion of reverse transcription, inhibit virus production, and reduce virus infectivity.
J Leukoc Biol. 1997 Jul;62(1):126-32. doi: 10.1002/jlb.62.1.126.

引用本文的文献

1
New Therapies and Strategies to Curb HIV Infections with a Focus on Macrophages and Reservoirs.新型疗法和策略以遏制 HIV 感染，重点关注巨噬细胞和病毒储存库。
Viruses. 2024 Sep 18;16(9):1484. doi: 10.3390/v16091484.
2
Animal models for studies of HIV-1 brain reservoirs.用于 HIV-1 脑库研究的动物模型。
J Leukoc Biol. 2022 Nov;112(5):1285-1295. doi: 10.1002/JLB.5VMR0322-161R. Epub 2022 Aug 31.
3
Crucial Role of Central Nervous System as a Viral Anatomical Compartment for HIV-1 Infection.中枢神经系统作为HIV-1感染的病毒解剖学隔室的关键作用。

本文引用的文献

1
Brain Macrophages in Simian Immunodeficiency Virus-Infected, Antiretroviral-Suppressed Macaques: a Functional Latent Reservoir.感染猿猴免疫缺陷病毒且接受抗逆转录病毒治疗抑制的猕猴中的脑巨噬细胞：一个功能性潜伏库
mBio. 2017 Aug 15;8(4):e01186-17. doi: 10.1128/mBio.01186-17.
2
HIV persistence in tissue macrophages of humanized myeloid-only mice during antiretroviral therapy.抗逆转录病毒治疗期间人源化纯髓系小鼠组织巨噬细胞中的HIV持续性。
Nat Med. 2017 May;23(5):638-643. doi: 10.1038/nm.4319. Epub 2017 Apr 17.
3
A mature macrophage is a principal HIV-1 cellular reservoir in humanized mice after treatment with long acting antiretroviral therapy.
Microorganisms. 2021 Dec 8;9(12):2537. doi: 10.3390/microorganisms9122537.
4
Microglia: The Real Foe in HIV-1-Associated Neurocognitive Disorders?小胶质细胞：HIV-1相关神经认知障碍中的真正敌人？
Biomedicines. 2021 Jul 30;9(8):925. doi: 10.3390/biomedicines9080925.
5
Predicted antiviral drugs Darunavir, Amprenavir, Rimantadine and Saquinavir can potentially bind to neutralize SARS-CoV-2 conserved proteins.预测的抗病毒药物达芦那韦、安普那韦、金刚烷胺和沙奎那韦可能会与新冠病毒保守蛋白结合以将其中和。
J Biol Res (Thessalon). 2021 Aug 4;28(1):18. doi: 10.1186/s40709-021-00149-2.
6
Important role of microglia in HIV-1 associated neurocognitive disorders and the molecular pathways implicated in its pathogenesis.小胶质细胞在 HIV-1 相关神经认知障碍中的重要作用及其发病机制中涉及的分子途径。
Ann Med. 2021 Dec;53(1):43-69. doi: 10.1080/07853890.2020.1814962. Epub 2020 Sep 17.
7
Mechanisms underlying of antiretroviral drugs in different cellular reservoirs with a focus on macrophages.以巨噬细胞为重点的不同细胞储存库中抗逆转录病毒药物的潜在机制。
Virulence. 2020 Dec;11(1):400-413. doi: 10.1080/21505594.2020.1760443.
8
Fosamprenavir with Ritonavir Pharmacokinetics during Pregnancy.孕期福沙那韦/利托那韦的药代动力学。
Antimicrob Agents Chemother. 2020 Mar 24;64(4). doi: 10.1128/AAC.02260-19.
9
Different Patterns of HIV-1 Replication in MACROPHAGES is Led by Co-Receptor Usage.不同的共受体使用模式导致巨噬细胞中 HIV-1 的复制情况不同。
Medicina (Kaunas). 2019 Jun 21;55(6):297. doi: 10.3390/medicina55060297.
在用长效抗逆转录病毒疗法治疗后的人源化小鼠中，成熟巨噬细胞是主要的HIV-1细胞储存库。
Retrovirology. 2017 Mar 9;14(1):17. doi: 10.1186/s12977-017-0344-7.
4
Tissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques.在未接受抗逆转录病毒治疗、感染 SIV 的亚洲猕猴中，组织驻留巨噬细胞可含有具有复制能力的病毒。
JCI Insight. 2017 Feb 23;2(4):e91214. doi: 10.1172/jci.insight.91214.
5
CD4/CD8 ratio and CD8 counts predict CD4 response in HIV-1-infected drug naive and in patients on cART.CD4/CD8 比值和 CD8 细胞计数可预测初治 HIV-1 感染患者及接受抗逆转录病毒治疗（cART）患者的 CD4 反应。
Medicine (Baltimore). 2016 Oct;95(42):e5094. doi: 10.1097/MD.0000000000005094.
6
Cell-to-Cell Spread of HIV and Viral Pathogenesis.HIV 细胞间传播与病毒发病机制。
Adv Virus Res. 2016;95:43-85. doi: 10.1016/bs.aivir.2016.03.001. Epub 2016 Apr 4.
7
Macrophages and HIV-1: An Unhealthy Constellation.巨噬细胞与HIV-1：一个不健康的组合。
Cell Host Microbe. 2016 Mar 9;19(3):304-10. doi: 10.1016/j.chom.2016.02.013.
8
Characterization, biomedical and agricultural applications of protease inhibitors: A review.蛋白酶抑制剂的表征、生物医学及农业应用：综述
Int J Biol Macromol. 2016 Oct;91:1120-33. doi: 10.1016/j.ijbiomac.2016.02.069. Epub 2016 Mar 5.
9
Viral infection: Playing hide and seek with HIV.病毒感染：与艾滋病毒捉迷藏
Nat Rev Microbiol. 2016 Mar;14(3):120. doi: 10.1038/nrmicro.2016.14. Epub 2016 Feb 8.
10
Persistent HIV-1 replication maintains the tissue reservoir during therapy.在治疗期间，持续的HIV-1复制维持着组织储存库。
Nature. 2016 Feb 4;530(7588):51-56. doi: 10.1038/nature16933. Epub 2016 Jan 27.