Liu Xuelai, Gao Peng, Du Juan, Zhao Xin, Wong Kenneth K Y
Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Department of Surgery, Harbin Children's Hospital, Harbin, China.
J Pediatr Surg. 2017 Dec;52(12):2083-2087. doi: 10.1016/j.jpedsurg.2017.08.026. Epub 2017 Sep 8.
In our previous study, we coated silver nanoparticles (AgNPs) onto the surface of absorbable braided suture using layer-by-layer deposition and demonstrated significant anti-inflammatory property during the early phase of intestinal anastomosis healing in mice. The present study aimed to further investigate the long-term anti-inflammatory efficacy.
AgNP-coated suture, antibiotic coated suture, and normal suture were respectively used for single layered, interrupted intestinal anastomosis. The anastomotic segments in each group were harvested on day 14, day 21, and day 28 postoperation and investigated for the degree of inflammation by cell infiltration and expression of cytokines as well as collagen deposition.
When compared with the control groups, the AgNP-coated suture group showed better histological appearance in the intestinal anastomotic segments at each time point. Immunohistochemistry staining and quantitative evaluation further indicated less macrophage infiltration and decreased production of IL-6, IL-10, and TNF-α (p<0.05). Masson staining showed normal collagen deposition and remodeling at intestinal anastomotic tissue in the AgNP-coated suture group.
Our study shows that AgNP-coated suture provides better long-term anti-inflammatory efficacy and ideal tissue remodeling in intestinal anastomosis. Despite these findings, clinical trials are still needed for evaluation before medical application.
在我们之前的研究中，我们通过逐层沉积法将银纳米颗粒（AgNPs）包覆在可吸收编织缝线表面，并证明其在小鼠肠吻合口愈合早期具有显著的抗炎特性。本研究旨在进一步探究其长期抗炎效果。
分别使用AgNP包覆缝线、抗生素包覆缝线和普通缝线进行单层间断肠吻合术。每组在术后第14天、第21天和第28天采集吻合段，通过细胞浸润、细胞因子表达以及胶原沉积来研究炎症程度。
与对照组相比，AgNP包覆缝线组在各时间点的肠吻合段组织学表现更佳。免疫组织化学染色和定量评估进一步表明巨噬细胞浸润减少，白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的产生减少（p<0.05）。Masson染色显示AgNP包覆缝线组肠吻合组织中的胶原沉积和重塑正常。
我们的研究表明，AgNP包覆缝线在肠吻合术中具有更好的长期抗炎效果和理想的组织重塑。尽管有这些发现，但在医学应用前仍需要进行临床试验评估。
