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[胡萝卜软腐欧文氏菌温和噬菌体59的重排DNA的限制图谱]

[Restriction map of permuted DNA of Erwinia carotovora temperate bacteriophage 59].

作者信息

Tovkach F I, Grizorian Iu A, Ruban V I, Danileĭchenko V V, Kishko Ia G

出版信息

Mol Gen Mikrobiol Virusol. 1988 Jan(1):20-4.

PMID:2895890
Abstract

The cyclic permutation and terminal redundancy were found in the genomes of Erwinia carotovora temperate bacteriophage 59 by electron microscopic studies. The headful mechanism for bacteriophage DNA cleavage and packaging during the phage morphogenesis was confirmed by the restriction analysis technique. Restriction map of the bacteriophage 59 DNA was constructed for restriction endonucleases BamHI, Bg1II, Eco31, Sa1I, SmaI, EcoRI.

摘要

通过电子显微镜研究发现,胡萝卜软腐欧文氏菌温和噬菌体59的基因组中存在环状排列和末端冗余。通过限制性分析技术证实了噬菌体形态发生过程中噬菌体DNA切割和包装的“满头部”机制。构建了噬菌体59 DNA针对限制性内切酶BamHI、Bg1II、Eco31、Sa1I、SmaI、EcoRI的限制性图谱。

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